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CCL3 correlates with ferroptosis in intervertebral disc degeneration and its prognostic significance

  • 0Department of Clinical Laboratory, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui Province, China.
Scientific Reports +

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April 9, 2025

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April 9, 2025

View abstract on PubMed

Summary

This summary is machine-generated.

CC Chemokine Ligand 3 (CCL3) shows promise as a diagnostic and prognostic biomarker for intervertebral disc degeneration (IVDD). This study reveals CCL3

Area Of Science

  • Biomedical research
  • Molecular biology
  • Cellular pathology

Background

  • Intervertebral disc degeneration (IVDD) is a common cause of low back pain, lacking early diagnostic markers.
  • Ferroptosis and iron homeostasis disturbances are implicated in IVDD pathogenesis.
  • CC Chemokine Ligand 3 (CCL3) role in IVDD progression and macrophage migration requires clarification.

Purpose Of The Study

  • To investigate the mechanisms of ferroptosis and CCL3 in IVDD.
  • To establish the diagnostic and prognostic significance of CCL3 in IVDD.
  • To identify potential biomarkers and therapeutic targets for IVDD.

Main Methods

  • Bioinformatic analysis of single-cell sequencing data from TCGA.
  • In vitro validation using human tissue samples.
  • Quantitative real-time polymerase chain reaction (qRT-PCR) and Western blotting.
  • Co-culture assays with neutralizing antibodies.

Main Results

  • CCL3 demonstrates potential as a diagnostic and prognostic biomarker for IVDD.
  • CCL3 modulates apoptosis via the pAMPK/AMPK pathway, influencing IVDD progression.
  • The study highlights CCL3's role in the pathogenesis of IVDD.

Conclusions

  • CCL3 is a promising biomarker for diagnosing and predicting the prognosis of IVDD.
  • Targeting CCL3 may offer novel therapeutic strategies for managing IVDD.
  • Further research is needed to fully explore CCL3-targeted therapies for IVDD.

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