An acidic microenvironment promotes lymphatic metastasis of melanoma by Thy-1 in endothelial cells and integrin αvβ3 in tumor cells
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View abstract on PubMed
Summary
This summary is machine-generated.The study reveals that an acidic tumor microenvironment promotes melanoma lymphatic metastasis. Acid-induced Thy-1 in lymphatic cells and integrin αvβ3 in melanoma cells enhance cell adhesion, driving metastasis.
Area Of Science
- Oncology
- Cell Biology
- Biochemistry
Background
- Melanoma tissues often present an acidic microenvironment.
- The impact of this acidity on melanoma's lymphatic metastasis remains largely unknown.
- Lymphatic metastasis is a critical factor in melanoma patient prognosis.
Purpose Of The Study
- To investigate the role of the acidic microenvironment in lymphatic endothelial cell function.
- To explore the mechanisms by which acidity influences melanoma cell metastasis.
Main Methods
- Gene expression profiling of human dermal lymphatic endothelial cells (HDLECs) under acidic conditions.
- Immunohistochemical analysis in a mouse melanoma model.
- In vitro cell adhesion assays and in vivo metastasis models.
Main Results
- Acidic conditions increased Thy-1 expression in HDLECs in a time-dependent manner.
- Integrin αvβ3 expression was upregulated in melanoma cells under acidic conditions.
- Acidic conditions enhanced HDLEC-melanoma cell adhesion, mediated by Thy-1 and integrin αvβ3, and silencing integrin αv reduced metastasis.
Conclusions
- Acid-induced Thy-1 in lymphatic endothelial cells and integrin αvβ3 in melanoma cells promote mutual adhesion.
- This interaction contributes significantly to melanoma's lymphatic metastasis.
- Targeting these interactions may offer therapeutic strategies for melanoma.
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