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Functional cobalt-doped hydrogel scaffold enhances concurrent vascularization and neurogenesis
Junqing Liu1,2, Jun Kang2, Ting Zou3
1Hospital of Stomatology, Guanghua School of Stomatology, Sun Yat-Sen University, Guangdong Provincial Key Laboratory of Stomatology, Guangzhou, China.
Journal of Nanobiotechnology
|April 9, 2025
Summary
This study developed a novel hydrogel scaffold that releases cobalt ions to promote blood vessel and nerve growth simultaneously, enhancing tissue regeneration.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Tissue Engineering
Background:
- Functional tissue regeneration requires coordinated vascular and nerve growth.
- Current strategies lack reliable methods for promoting both vasculogenesis and neurogenesis concurrently.
Purpose of the Study:
- To develop a 3D hydrogel scaffold (GelMA-MWCNTs/Co) for controlled cobalt ion release.
- To investigate its dual effects on promoting vasculogenesis and neurogenesis for enhanced tissue repair.
Main Methods:
- Fabrication of a 3D gelatin methacryloyl-multi-walled carbon nanotube/cobalt (GelMA-MWCNTs/Co) hydrogel.
- Assessment of sustained cobalt ion release and its effect on cell viability.
- Evaluation of hydrogel's impact on human umbilical vein endothelial cells (HUVECs) vasculogenesis and stem cells from apical papilla (SCAP) neurogenesis.
- In vivo studies to analyze neovascularization and neural differentiation.
Main Results:
- The GelMA-MWCNTs/Co hydrogel demonstrated sustained cobalt ion release, supporting cell viability.
- Enhanced vasculogenesis of HUVECs co-cultured with SCAP was observed.
- Facilitated interaction between vascular and neural structures derived from SCAP.
- In vivo studies showed improved neovascularization and host vasculature anastomosis.
- Pre-vascularized scaffolds promoted neural differentiation of SCAP in regenerated tissue.
Conclusions:
- The developed GelMA-MWCNTs/Co hydrogel effectively promotes simultaneous vascularization and neurogenesis.
- Integrating cobalt ion release materials with dental-derived stem cells in hydrogels shows promise for complex tissue regeneration.
