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Intralipid infusion in neonates: effects on polymorphonuclear leukocyte function.

J G Wheeler, R J Boyle, J S Abramson

    Journal of Pediatric Gastroenterology and Nutrition
    |June 1, 1985
    PubMed
    Summary

    Intralipid (IL) infusion at 1 g/kg/24 h did not affect neonatal polymorphonuclear leukocyte (PMNL) function in vitro or in vivo. This suggests slow IL infusion is safe for neonatal PMNL oxidative function.

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    Area of Science:

    • Neonatal immunology
    • Lipid emulsion research
    • Cellular function studies

    Background:

    • Neonatal polymorphonuclear leukocytes (PMNLs) play a crucial role in immune defense.
    • Intralipid (IL), a common lipid emulsion, is used in parenteral nutrition.
    • Potential effects of IL on neonatal immune function require thorough investigation.

    Purpose of the Study:

    • To evaluate the impact of Intralipid (IL) on neonatal polymorphonuclear leukocyte (PMNL) function.
    • To assess both in vitro and in vivo effects of IL on PMNL chemotactic (CT) and chemiluminescent (CL) activities.

    Main Methods:

    • In vitro studies involved incubating cord and adult PMNLs with IL (10 mg/ml).
    • In vivo studies monitored PMNL CL activity in 14 neonates before and after IL infusion (1 g/kg/24 h).

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  • Paired controls incubated with buffer were used for in vitro comparisons.

    • Main Results:

    • No significant abnormalities in PMNL chemotactic (CT) or chemiluminescent (CL) activities were observed in vitro when PMNLs were incubated with IL.
    • In vivo, postinfusion CL activity of PMNLs in neonates did not significantly differ from preinfusion levels.
    • Slow IL infusion (1 g/kg/24 h) showed no detectable alteration in neonatal PMNL oxidative function.

    Conclusions:

    • Intralipid (IL) infusion at a rate of 1 g/kg/24 h does not impair neonatal PMNL oxidative function.
    • Current findings suggest the safety of slow IL infusion for neonatal immune cell function.
    • Further research is needed to confirm the safety of long-term therapy with higher doses of IL.