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Enzymatic Isolation of Skeletal Muscle Interstitial Extracellular Vesicles
Published on: February 7, 2025
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Extracellular Vesicles Released From Skeletal Muscle Post-Chronic Contractile Activity Increase Mitochondrial
Patience O Obi1,2,3,4,5, Tamiris F G Souza1,2,4,5, Berkay Özerkliğ1,2,4,5,6
1Diabetes Research Envisioned and Accomplished in Manitoba (DREAM) Research Theme, Winnipeg, Manitoba, Canada.
Journal of Extracellular Vesicles
|April 10, 2025
Summary
Chronic contractile activity (CCA) increases skeletal muscle extracellular vesicles (Skm-EVs) that enhance mitochondrial biogenesis in recipient cells, mimicking exercise benefits.
Area of Science:
- Cell Biology
- Exercise Physiology
- Extracellular Vesicles
Background:
- Skeletal muscle extracellular vesicles (Skm-EVs) role in exercise adaptation is unclear.
- Distinguishing Skm-EVs from exercising muscle in vivo is challenging.
Purpose of the Study:
- Investigate the impact of chronic contractile activity (CCA) on Skm-EVs.
- Determine the functional effects of CCA-induced Skm-EVs on recipient cells.
Main Methods:
- Differentiated myoblasts were subjected to electrical stimulation (CCA).
- Skm-EVs were isolated using differential ultracentrifugation and characterized (TRPS, TEM, Western Blot).
- Recipient C2C12 myoblasts were treated with Skm-EVs to assess functional effects.
Main Results:
- CCA increased Skm-EV secretion and concentration, with higher enrichment of small-EVs and specific markers (CD81, Tsg101, HSP70).
- CCA-Skm-EVs enhanced mitochondrial biogenesis, oxygen consumption rates, and ATP production in recipient myoblasts.
- Proteinase K treatment abrogated the pro-metabolic effect, suggesting a protein-mediated mechanism.
Conclusions:
- CCA-induced Skm-EVs transmit exercise-associated pro-metabolic effects to recipient cells.
- These findings highlight a novel role for Skm-EVs in mediating cellular adaptations to exercise.
- Further research is needed to elucidate the specific mechanisms involved.
Keywords:
chronic contractile activitydifferential ultracentrifugationextracellular vesiclesmitochondrial biogenesismyoblastsmyotubesskeletal muscle cellsMore Related Videos
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