Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mechanical Flexibility Enables DNA Origami to Overcome Steric Confinement in Mucus.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Light-switchable swarming of biohybrid microrobots.

Science advances·2026
Same author

SERS-Based Nano- and Microsystems Toward Biomedical Applications.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

Method of oral delivery affects vitamin C-mediated alleviation of colitis in a mouse model.

Gut microbes reports·2026
Same author

Fungal Mycelium Films Engineered as Renewable Fibrous Materials for Drug Delivery.

ACS applied materials & interfaces·2026
Same author

Corrigendum to "Preparation of a universally usable, animal product free, defined medium for 2D and 3D culturing of normal and cancer cells" [MethodsX 12 (2024) 102592].

MethodsX·2026

Related Experiment Video

Updated: May 15, 2025

Enhanced Viability for Ex vivo 3D Hydrogel Cultures of Patient-Derived Xenografts in a Perfused Microfluidic Platform
12:12

Enhanced Viability for Ex vivo 3D Hydrogel Cultures of Patient-Derived Xenografts in a Perfused Microfluidic Platform

Published on: December 5, 2020

6.0K

Advancing humanized 3D tumor modeling using an open access xeno-free medium.

Atena Malakpour-Permlid1, Manuel Marcos Rodriguez1, Kinga Zór1,2

  • 1Center for Intelligent Drug Delivery and Sensing Using Microcontainers and Nanomechanics (IDUN), Department of Health Technology, Technical University of Denmark, Lyngby, Denmark.

Frontiers in Toxicology
|April 10, 2025
PubMed
Summary

This study successfully adapted cancer cells and fibroblasts to an animal-product-free medium for advanced 3D cell culture. This new system enhances 3D drug screening by using a chemically defined medium, improving in vitro models.

Keywords:
3D cell cultureFBS-free mediumHeLa cancer cellsMCF-7 cancer cellsOredsson universal replacement (OUR) mediumcancer-associated fibroblasts (CAFs)high throughput drug screeningpaclitaxel

More Related Videos

Generation of Microtumors Using 3D Human Biogel Culture System and Patient-derived Glioblastoma Cells for Kinomic Profiling and Drug Response Testing
09:24

Generation of Microtumors Using 3D Human Biogel Culture System and Patient-derived Glioblastoma Cells for Kinomic Profiling and Drug Response Testing

Published on: June 9, 2016

9.0K
Author Spotlight: Recreating Melanoma Complexity with Patient-Derived Organoids for Immunotherapy Evaluation
05:33

Author Spotlight: Recreating Melanoma Complexity with Patient-Derived Organoids for Immunotherapy Evaluation

Published on: September 6, 2024

1.2K

Related Experiment Videos

Last Updated: May 15, 2025

Enhanced Viability for Ex vivo 3D Hydrogel Cultures of Patient-Derived Xenografts in a Perfused Microfluidic Platform
12:12

Enhanced Viability for Ex vivo 3D Hydrogel Cultures of Patient-Derived Xenografts in a Perfused Microfluidic Platform

Published on: December 5, 2020

6.0K
Generation of Microtumors Using 3D Human Biogel Culture System and Patient-derived Glioblastoma Cells for Kinomic Profiling and Drug Response Testing
09:24

Generation of Microtumors Using 3D Human Biogel Culture System and Patient-derived Glioblastoma Cells for Kinomic Profiling and Drug Response Testing

Published on: June 9, 2016

9.0K
Author Spotlight: Recreating Melanoma Complexity with Patient-Derived Organoids for Immunotherapy Evaluation
05:33

Author Spotlight: Recreating Melanoma Complexity with Patient-Derived Organoids for Immunotherapy Evaluation

Published on: September 6, 2024

1.2K

Area of Science:

  • Cell Biology
  • Biotechnology
  • Drug Discovery

Background:

  • Traditional in vitro cell culture often uses animal-derived components like fetal bovine serum (FBS), posing challenges such as poor microenvironment mimicry and contamination risks.
  • Advanced three-dimensional (3D) models offer better in vivo mimicry but have seen limited use with animal-product-free media.
  • There is a need for more realistic and reproducible in vitro models for drug screening and toxicity testing.

Purpose of the Study:

  • To develop a more realistic 3D in vitro drug screening system by combining a high-throughput 3D model with an animal-product-free, chemically defined medium.
  • To adapt human cancer cell lines (HeLa, MCF-7) and cancer-associated fibroblasts (CAFs) to the Oredsson Universal Replacement (OUR) medium.
  • To evaluate the performance of the adapted cells in 3D culture and assess drug toxicity using paclitaxel (PTX).

Main Methods:

  • Gradual adaptation of HeLa, MCF-7, and CAFs from FBS-supplemented medium to OUR medium.
  • Monitoring of cell attachment, proliferation, and morphology using phase contrast and real-time live imaging.
  • Assessment of cell behavior in 3D fiber scaffolds and evaluation of paclitaxel (PTX) drug toxicity.

Main Results:

  • Successful adaptation of HeLa, MCF-7, and CAFs to OUR medium, maintaining sustained growth kinetics and population doubling times.
  • Morphological changes observed in HeLa and MCF-7 cells (more spread-out cytoplasm, lower circularity), while CAFs remained unaffected.
  • Efficient cell distribution, infiltration, and expansion within 3D fiber scaffolds when cultured in OUR medium.
  • Cells in 3D cultures with OUR medium showed reduced sensitivity to paclitaxel (PTX), consistent with FBS-supplemented cultures.

Conclusions:

  • The combination of a high-throughput 3D model and OUR medium provides a more realistic in vitro system for drug screening.
  • Animal-product-free media can be successfully used for advanced 3D cell culture and toxicity testing.
  • This approach encourages the adoption of animal-product-free formulations in research and drug development to improve in vitro model reliability.