Related Experiment Video

Updated: May 15, 2025

High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture 4C-seq
09:06

High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture 4C-seq

Published on: October 5, 2018

10.2K

Comprehensive genome analysis of Streptomyces caeruleatus S14 isolated from rice rhizosphere

Sucharita Mohapatra1,2, S R Prabhukarthikeyan1, Gayatri Biswal2

  • 1ICAR-National Rice Research Institute, Cuttack, India.

Frontiers in Plant Science
|April 10, 2025
PubMed

Insights

Streptomyces caeruleatus strain S14 effectively inhibits rice blast fungus Magnaporthe oryzae. This study presents the first whole genome sequence of this potent biocontrol agent from Indian rice fields.

Area of Science:

  • Microbiology
  • Plant Pathology
  • Genomics

Background:

  • Rice blast, caused by Magnaporthe oryzae, is a major threat to global rice production.
  • Biological control agents offer a sustainable alternative to chemical fungicides for disease management.

Purpose of the Study:

  • To evaluate the efficacy of Streptomyces spp. against Magnaporthe oryzae.
  • To perform whole genome sequencing of the most effective Streptomyces strain for understanding its biocontrol mechanisms.

Main Methods:

  • Screening of Streptomyces isolates for inhibition of Magnaporthe oryzae mycelial growth.
  • Identification of the effective strain using 16S rRNA sequencing.
  • Whole genome sequencing using Illumina NovaSeq 6000 and subsequent annotation (RASTtk, PATRIC).

Main Results:

  • Streptomyces caeruleatus strain S14 showed significant inhibition (74.7%) of Magnaporthe oryzae mycelial growth.
  • The complete genome of S. caeruleatus S14 was sequenced (9.7 Mb) and annotated, revealing 9,191 protein-coding sequences.
  • The genome analysis identified genes associated with metabolism, defense, and antimicrobial activity.

Conclusions:

  • Streptomyces caeruleatus S14 is a highly effective biocontrol agent against rice blast.
  • The genome sequence provides valuable genetic insights into the antimicrobial properties of S. caeruleatus S14.
  • This is the first report of a whole genome sequence for S. caeruleatus isolated from rice rhizosphere in India, paving the way for further research and application.
Keywords:
Streptomyces caeruleatusactinobacteriabiocontrolgenome annotationgenome sequencerice blastwhole genome sequence

More Related Videos

Ultra-long Read Sequencing for Whole Genomic DNA Analysis10:34

Ultra-long Read Sequencing for Whole Genomic DNA Analysis

Published on: March 15, 2019

22.6K
Genome-wide Profiling of Transcription Factor-DNA Binding Interactions in Candida albicans: A Comprehensive CUT&RUN Method and Data Analysis Workflow07:48

Genome-wide Profiling of Transcription Factor-DNA Binding Interactions in Candida albicans: A Comprehensive CUT&RUN Method and Data Analysis Workflow

Published on: April 1, 2022

4.0K

Related Concept Videos

Genomics02:02

Genomics

Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
35.3K
Evolutionary Relationships through Genome Comparisons02:54

Evolutionary Relationships through Genome Comparisons

Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...
5.6K
Genome Annotation and Assembly03:36

Genome Annotation and Assembly

The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
18.7K
Genome-wide Association Studies-GWAS01:11

Genome-wide Association Studies-GWAS

Genome-wide association studies or GWAS are used to identify whether common SNPs are associated with certain diseases. Suppose specific SNPs are more frequently observed in individuals with a particular disease than those without the disease. In that case, those SNPs are said to be associated with the disease. Chi-square analysis is performed to check the probability of the allele likely to be associated with the disease.
GWAS does not require the identification of the target gene involved in...
12.2K