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In Vitro Comparative Study of Calcium Hydroxyapatite (Stiim): Conventional Saline Dilution Versus Poly-Micronutrient
Valeria Dal Col1, Cassiano Marchi2, Fabio Ribas1
1Dentistry, Protocoll Clinique, Vitória, BRA.
Cureus
|April 10, 2025
Summary
Calcium hydroxylapatite (CaHA) with poly-micronutrients (CaHA/PMN) significantly boosts skin cell viability and collagen production more than standard CaHA. This combination enhances skin rejuvenation and elasticity.
Area of Science:
- Biomaterials Science
- Dermatology
- Regenerative Medicine
Background:
- Calcium hydroxylapatite (CaHA) is a well-established aesthetic treatment used for dermal filling and stimulating collagen production.
- Enhancing the biostimulatory effects of CaHA could lead to improved skin rejuvenation and elasticity.
Purpose of the Study:
- To compare the efficacy of CaHA diluted in saline versus CaHA mixed with a poly-micronutrient solution (CaHA/PMN).
- To evaluate the impact of CaHA/PMN on fibroblast cell viability and the expression of key regenerative genes.
Main Methods:
- Primary human fibroblasts were treated with CaHA/saline and CaHA/PMN in an in vitro setting.
- Assessed cell viability, type I collagen, elastin, FOXO3, SIRT-1, and SIRT-3 gene expression.
Main Results:
- Both CaHA formulations improved cellular responses compared to the control.
- CaHA/PMN demonstrated superior results, significantly increasing cell viability, collagen, and elastin synthesis.
- CaHA/PMN also showed greater upregulation of SIRT-1, SIRT-3, and FOXO3 gene expression.
Conclusions:
- Poly-micronutrient solutions can potentiate the regenerative capabilities of CaHA.
- CaHA/PMN presents a promising strategy for enhancing skin rejuvenation and improving skin elasticity.
