Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Capillary Electrophoresis: Applications

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

<i>Pseudomonas aeruginosa</i> infection causes lysosomal dysfunction in the cystic fibrosis bronchial epithelium.

Infection and immunity·2026

Same author

Pilot and Feasibility Study of an Individualized Telehealth Exercise Program for People with Cystic Fibrosis.

Journal of functional morphology and kinesiology·2026

Same author

Special Issue "Inflammatory Airway Diseases: Diagnosis, Pathology, Molecular Mechanisms and Treatment Options".

International journal of molecular sciences·2026

Same author

Epithelial extracellular vesicles induce inflammation and neutrophil activation in the <i>Pseudomonas aeruginosa</i> infected cystic fibrosis bronchial epithelium.

Frontiers in immunology·2026

Same author

Social determinants of health, cystic fibrosis-related diabetes, and mental health in adult patients with cystic fibrosis: a critical synthesis of narrative review.

Therapeutic advances in respiratory disease·2026

Same author

Assessment of efficacy and tolerability of elexacaftor-tezacaftor-ivacaftor in an observational cohort study of "aged" people with cystic fibrosis.

Therapeutic advances in respiratory disease·2026

Search research articles

Related Experiment Video

Updated: May 13, 2026

Using Unfixed, Frozen Tissues to Study Natural Mucin Distribution

Using Unfixed, Frozen Tissues to Study Natural Mucin Distribution

Published on: September 21, 2012

Simple and accessible methods for quantifying isolated mucins for further evaluation.

Hannah J McIntire-Ray^1,2, Elex S Rose^1,2, Stefanie Krick^1,2

¹Gregory Fleming James Cystic Fibrosis Research Center, Univ. of Alabama at Birmingham, Birmingham, AL, USA.

|April 10, 2025

Summary

This summary is machine-generated.

This study details a workflow for isolating and quantifying mucin proteins, like MUC5B, from saliva. Using a bovine submaxillary mucin standard improved quantitation accuracy by nearly twofold for mucin studies.

Keywords:

Ag-PAGE Agarose-polyacrylamide Gel electrophoresis Isolation, Quantitation, and Evaluation of Salivary MUC5B Isopycnic ultracentrifugation Mucin biology Mucin quantitation Salivary MUC5B

More Related Videos

Mucin Agarose Gel Electrophoresis: Western Blotting for High-molecular-weight Glycoproteins

Mucin Agarose Gel Electrophoresis: Western Blotting for High-molecular-weight Glycoproteins

Published on: June 14, 2016

Noninvasive Sampling of Mucosal Lining Fluid for the Quantification of In Vivo Upper Airway Immune-mediator Levels

Noninvasive Sampling of Mucosal Lining Fluid for the Quantification of In Vivo Upper Airway Immune-mediator Levels

Published on: August 7, 2017

Related Experiment Videos

Last Updated: May 13, 2026

Using Unfixed, Frozen Tissues to Study Natural Mucin Distribution

Using Unfixed, Frozen Tissues to Study Natural Mucin Distribution

Published on: September 21, 2012

Mucin Agarose Gel Electrophoresis: Western Blotting for High-molecular-weight Glycoproteins

Mucin Agarose Gel Electrophoresis: Western Blotting for High-molecular-weight Glycoproteins

Published on: June 14, 2016

Noninvasive Sampling of Mucosal Lining Fluid for the Quantification of In Vivo Upper Airway Immune-mediator Levels

Noninvasive Sampling of Mucosal Lining Fluid for the Quantification of In Vivo Upper Airway Immune-mediator Levels

Published on: August 7, 2017

Area of Science:

Biochemistry
Proteomics
Mucinology

Background:

Mucins are critical glycoproteins in mucosal secretions.
Accurate isolation and quantitation of mucins are essential for understanding their roles in health and disease.

Purpose of the Study:

To establish a detailed workflow for mucin protein isolation, quantitation, and evaluation.
To present methods applicable to various mucin-containing biological samples, focusing on MUC5B from the respiratory system.

Main Methods:

A streamlined, one-step isopycnic ultracentrifugation was employed to isolate MUC5B from human saliva.
A modified Bradford protein assay using a bovine submaxillary mucin (BSM) standard was developed for accurate mucin quantitation.
Agarose-polyacrylamide gel electrophoresis was used for visualization and confirmation of mucin isolation and quantitation.

Main Results:

The study successfully isolated and quantified MUC5B from human saliva.
Quantitation using the BSM standard yielded a near 2-fold increase compared to a bovine serum albumin (BSA) standard, highlighting the importance of a representative standard.
The developed methods confirmed mucin isolation and quantitation.

Conclusions:

The presented workflow provides a robust method for mucin analysis in various biological samples.
The findings underscore the significance of using appropriate standards, like BSM, for accurate mucin quantitation.
These techniques advance mucin studies and understanding of mucin implications in health and disease.