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Updated: May 15, 2025

Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments
Published on: February 7, 2025
Current issues of tandem mass spectrum (MS2)-based glycoproteomics and efforts to complement them
1Institute for Glyco-core Research (iGCORE), Nagoya University, Japan.
Abstract:
With the development of liquid chromatography/mass spectrometers that support proteomics and the associated development of analytical methods and data analysis software, the number of proteins that can be identified and quantified in a single analysis is approaching the level of transcriptomics. However, many problems remain to be solved in protein glycosylation analysis. This mini-review discusses the technical issues of MS2-based glycoprotein identification and efforts to complement them by the Human Glycome Atlas (HGA) Project in Japan.
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