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Super-resolution Fluorescence Microscopy

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Updated: May 15, 2025

Using Flatbed Scanners to Collect High-resolution Time-lapsed Images of the Arabidopsis Root Gravitropic Response

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Published on: January 25, 2014

Root expansion microscopy: A robust method for super resolution imaging in Arabidopsis.

Magali S Grison¹, Guillaume Maucort^2,3, Amandine Dumazel¹

¹Laboratoire de Biogenèse Membranaire, UMR5200, CNRS, Université de Bordeaux, 33140 Villenave d'Ornon, France.

|April 10, 2025

Summary

ROOT-ExM is a new expansion microscopy method for plant tissues. It enables nanoscale imaging of plant cell structures, overcoming previous limitations in plant biology research.

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Last Updated: May 15, 2025

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Published on: January 25, 2014

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Published on: May 14, 2020

Area of Science:

Plant Biology
Microscopy
Cell Biology

Background:

Expansion microscopy (ExM) enables super-resolution imaging by physically enlarging samples.
Existing ExM methods are not optimized for the unique challenges of plant tissues.
Super-resolution imaging in plants is crucial for understanding cellular processes.

Purpose of the Study:

To develop and validate a novel ExM technique for plant tissues.
To achieve nanoscale visualization of plant subcellular structures.
To overcome limitations in plant super-resolution microscopy.

Main Methods:

ROOT-ExM: A new expansion microscopy protocol for plant tissues.
Utilized immunolocalization, compartment-specific dyes, and native fluorescence.
Combined ROOT-ExM with STED and lattice light sheet microscopy.

Main Results:

ROOT-ExM provides isotropic 4-fold expansion, enhancing resolution.
Successfully imaged organelles (Golgi, ER), cytoskeleton, and plasmodesmata.
Achieved unprecedented resolution of plasmodesmata and enabled 3D quantitative analysis of cellular processes.

Conclusions:

ROOT-ExM is an effective and cost-efficient solution for plant super-resolution imaging.
This method significantly advances the study of plant subcellular architecture.
Opens new avenues for nanoscale investigations in plant biology.