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Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates

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Published on: March 20, 2015

Semiconductor Superstructures with Multiple Synergistic Resonances for SERS Exploring Multiplex Noncovalent

Jiaojiao Zhao¹, Lei Sun¹, Yumiao Dong²

¹College of Chemistry, Chemical Engineering and Resource Utilization, Northeast Forestry University, Harbin 150040, P. R. China.

|April 10, 2025

Summary

This summary is machine-generated.

This study introduces a new method using semiconductor surface-enhanced Raman scattering (SERS) to identify multiple noncovalent interactions (NCIs) between molecules. This technique advances the study of biological interactions and chemical bonding.

Keywords:

TiO2 superstructures charge-transfer multiple synergistic resonances noncovalent interactions semiconductor SERS

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Area of Science:

Chemistry
Spectroscopy
Materials Science

Background:

Noncovalent interactions (NCIs) are fundamental to biological processes and chemical applications.
Surface-enhanced Raman scattering (SERS) spectroscopy is a powerful tool for molecular analysis.
Developing sensitive SERS substrates is crucial for detecting subtle molecular interactions.

Purpose of the Study:

To demonstrate the identification of multiple NCIs between interacting species using semiconductor-based SERS.
To develop a SERS system with enhanced activity and interfacial charge-transfer sensitivity.
To explore the application of this method in analyzing DNA bases and sequences.

Main Methods:

Design and fabrication of submicrometer-sized TiO2 superstructures for synergistic SERS enhancement.
Utilizing the TiO2/4-mercaptobenzoic acid (MBA) system for high SERS activity and charge-transfer sensitivity.
Analysis of vibrational frequency shifts in MBA to correlate with various intermolecular interactions (hydrogen-bonding, ionic).
SERS analysis of noncovalent interactions for DNA bases and single-stranded DNA sequences.
Proof-of-concept study using relative SERS intensity to detect base content in DNA.

Main Results:

The designed TiO2 superstructures exhibited synergistic Mie and charge-transfer resonances for enhanced SERS.
MBA vibrational frequencies shifted demonstrably with different intermolecular interactions.
Multiple NCIs were successfully identified in DNA bases and sequences.
Relative SERS intensity was shown to be effective in quantifying base content in DNA.

Conclusions:

Semiconductor-based SERS can effectively identify multiple noncovalent interactions between species.
The developed TiO2/MBA system offers high sensitivity for probing intermolecular interactions.
This approach holds significant potential for applications in studying molecular interactions and DNA analysis.