Related Experiment Video
Updated: May 15, 2025
Murine Fecal Isolation and Microbiota Transplantation
Published on: May 26, 2023
Single Enema Fecal Microbiota Transplantation in Cats With Chronic Enteropathy
Dimitra A Karra1, Jan S Suchodolski2, Shelley J Newman3
1Clinic of Medicine, Faculty of Veterinary Science, University of Thessaly, Karditsa, Greece.
Background:
Chronic enteropathies (CE) are common in cats, and alterations of the intestinal microbiota might be involved in the pathogenesis.
Hypothesis/Objectives:
To evaluate the efficacy of a single enema fecal microbiota transplantation (FMT) in improving intestinal dysbiosis and clinical scores in cats with CE.
Animals:
Twenty-eight cats with either chronic inflammatory enteropathy (CIE; n = 19) or small cell gastrointestinal lymphoma (SCGL; n = 9) were prospectively enrolled.
Methods:
Eleven cats were randomly selected to receive a single enema FMT (FMT-group), and 17 cats were used as controls. Clinical activity was determined using the Feline Chronic Enteropathy Activity Index (FCEAI), and intestinal dysbiosis was determined using the feline dysbiosis index (DI) on the day of FMT (T0) and 30 days after FMT (T1).
Results:
At T0, 14/28 cats had an abnormal DI > 0. No significant difference was found in the DI from T0 to T1 in the FMT group (mean[SD]: 0.01[2.5] vs. 0.7[2.1]; p = 0.47). No significant difference was found in the DI between the FMT group and the control group at T1 (mean[SD]: -0.7[2.1] vs. 0.8[1.8]; p = 0.92). FCEAI significantly decreased at T1 compared to T0 in the FMT group (median[IQR] 10.0[7.7-11.3] vs. 4.5[4-5]; p = 0.002). No significant difference was found in the FCEAI between the FMT group and the control group at T1 (median[IQR] 4.5[4-5] vs. 4[3-5.75]; p = 0.64).
Conclusions:
In this study, single enema FMT did not lead to a significant improvement in DI or FCEAI in cats with CE compared to controls.
