The transcription factors PIF4 and PIF5 interact with WRINKLED1 to modulate fatty acid biosynthesis during seed maturation
Huimei Liao1, Bojing Feng1, Mengrui Wen1
1Guangdong Key Laboratory of Biotechnology for Plant Development, College of Life Sciences, South China Normal University, Guangzhou 510631, China.
Abstract:
PHYTOCHROME-INTERACTING FACTORS (PIFs), members of the basic helix-loop-helix (bHLH) transcription factor (TF) family, regulate various developmental processes in Arabidopsis (Arabidopsis thaliana). However, their involvement in fatty acid biosynthesis and seed maturation is largely unknown. WRINKLED1 (WRI1) is a pivotal TF regulating plant fatty acid biosynthesis. In this study, we identified WRI1 as an interacting partner of PIF4 and PIF5. PIF4 and PIF5, similar to WRI1, are expressed during seed maturation. Over-expressing PIF4 or PIF5 triggers increased seed fatty acid biosynthesis, while loss-of-function pif4 and pif5 mutants exhibit reduced seed fatty acid biosynthesis and delayed seed maturation. Further analysis revealed that PIF4 and PIF5 promote WRI1-dependent expression of fatty acid biosynthesis genes. Together, our findings suggest that PIF4 and PIF5 interact with WRI1, thereby promoting fatty acid biosynthesis gene expression and increasing fatty acid biosynthesis. This study reports a previously uncharacterized regulatory mechanism that fine-tunes seed fatty acid biosynthesis and seed maturation.
More Related Videos
10:29Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput
Published on: March 30, 2018
07:34The Power of Simplicity: Sea Urchin Embryos as in Vivo Developmental Models for Studying Complex Cell-to-cell Signaling Network Interactions
Published on: February 16, 2017
Related Concept Videos
Regulation of the Unfolded Protein Response
Non-Canonical Wnt Signaling Pathways
Canonical Wnt Signaling Pathway
Master Transcription Regulators
Seed Structure and Early Development of the Sporophyte
Regulation of Expression at Multiple Steps
