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Identification of Adult Attractants through Functional Analyses of Chemosensory Protein 21 from the Bean Bug
Nan Gu1, Xin-Yao Sun1, Zhan-Qi Li1
1Anhui Engineering Research Center for Green Production Technology of Drought Grain Crops, Anhui Province Key Laboratory of Pollutant Sensitive Materials and Environmental Remediation, Huaibei City Key Laboratory of Green Prevention and Control of Crop Diseases and Pests, College of Life Sciences, Huaibei Normal University, Huaibei 235000, China.
Abstract:
Chemosensory proteins (CSPs) play a key role in olfactory recognition, offering promising avenues for the development of innovative insect pest management strategies. In this study, we investigated RpedCSP21 from the bean bug Riptortus pedestris, a key legume pest species, and first confirmed RpedCSP21 displayed antennal-skewed expression. Fluorescence competitive binding assays, structural modeling, molecular docking, and site-directed mutagenesis revealed that RpedCSP21 exhibits certain binding affinity for five soybean volatiles and one aggregation pheromone. RNA interference (RNAi) successfully silenced the RpedCSP21 gene, resulting in significantly reduced electroantennography (EAG) responses to these ligands compared to control individuals. Behavioral assays in a Y-tube olfactometer further confirmed weakened attraction to the six ligands, consistent with the EAG findings. These results indicate that RpedCSP21 is integral to the chemical perception of R. pedestris. Furthermore, the use of bacteria-expressed dsRNA delivery systems highlights the potential of targeting RpedCSP21 for the development of eco-friendly population management strategies.
