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Standard Preparation Protocol of Human Skin and Skin Substitute Samples for Transmission Electron Microscopy
Gery Barmettler1, Urs Ziegler2
1Center for Microscopy and Image Analysis, University of Zurich, Zurich, Switzerland.
Methods in Molecular Biology (Clifton, N.J.)
|April 10, 2025
Abstract:
This chapter is intended to be a guide to skin and skin substitutes studies dealing with the analysis of subcellular structures of cells and tissues by providing an exhaustive sample preparation protocol for Transmission Electron Microscopy (TEM). Critical steps to obtain good ultrastructure are mentioned as well as notes to improve the sample collection. New ways to automatize the imaging acquisition are discussed.
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