Microbial community analysis of the biofilms of both working and counter electrodes in single-chamber microbial electrolysis cells

This study was conducted to delineate microbial community development and composition on both working and counter electrodes in single-chamber microbial electrolysis cells (MECs) using synthetic wastewater. Two separate bioelectrochemical reactors were inoculated with anaerobic sludge. The first was operated at an anodic potential poised at + 0.4 V and the second one at a cathodic potential poised at -0.7 V, both vs. an Ag/AgCl reference electrode. The performance of the MECs, including current generation, bioelectrochemical activity of the biofilms on both the working and counter electrodes, and chemical oxygen demand (COD) depletion were monitored over the last 45 days of operation. Scanning electron microscopy (SEM) and 16S rRNA gene sequencing were performed to delineate the development and morphology of the microbial communities on both the working and the counter electrodes. The current generated at the anodic working electrode provided evidence of the growth of anode-respiring exoelectrogens (Clostridium sensu stricto). Similarly, the Faradaic current data at the cathodic working electrode confirmed the formation of an electroactive biofilm dominated by acetoclastic and hydrogenotrophic methanogens (Methanothrix and Methanobacterium). Microbial communities on the counter electrodes were found to be richer but less diverse compared to those on the working electrodes. These communities were likely influenced by the fluctuating potentials at the counter electrodes. SEM observations were consistent with the microbial analysis. These findings demonstrate the ability of a mixed inoculum to shift towards anode-reducing and cathode methanogenic communities using a complex substrate on a constant working electrode and varying counter electrode potentials.