Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Synthesis and Regulation of Thyroid Hormones01:20

Synthesis and Regulation of Thyroid Hormones

4.0K
Low blood levels of the thyroid hormones — triiodothyronine (T3) and thyroxine (T4) — signal the hypothalamus to release the thyrotropin-releasing hormone (TRH). TRH then reaches the pituitary gland and stimulates the release of thyroid-stimulating hormone(TSH) into the bloodstream.
Upon reaching the thyroid gland, TSH stimulates the follicular cells' active uptake of iodide ions from the blood. The ions diffuse to the apical surface of the cells and are oxidized to iodine. The...
4.0K
Functions of Thyroid Hormones01:18

Functions of Thyroid Hormones

2.4K
The thyroid hormone (TH) plays a pivotal role in the intricate orchestration of physiological processes, exerting profound effects on development, metabolism, and homeostasis throughout different life stages.
TH is indispensable for the normal development and maturation of the skeletal, muscular, and nervous systems during fetal and childhood growth. It facilitates bone mineral turnover and regulates protein synthesis in developing tissues, contributing significantly to overall growth and...
2.4K
The Thyroid Gland01:23

The Thyroid Gland

3.4K
The thyroid gland is a small, butterfly-shaped gland located in the neck and covers the anterior surface of the trachea. The gland has two lateral lobes connected by a thin tissue mass called the isthmus. Internally, each lobe comprises many small spherical structures known as thyroid follicles, surrounded by a network of blood vessels.
The follicles have a central cavity lined by simple cuboidal to squamous epithelial cells called follicular cells. These cells produce the glycoprotein...
3.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Clinical Application of RDW in Predicting the Severity of Liver Cirrhosis.

Clinical laboratory·2026
Same author

Design, Synthesis and Biological Evaluation of Aminoalkylated Paeonol Chalcone Derivatives.

Chemical biology & drug design·2026
Same author

Shell-isolated nanoparticle-enhanced femtosecond stimulated Raman spectroscopy reveals ultrafast molecular dynamics of surface reactions.

Nature communications·2025
Same author

The Role of TNF - α, IFN - γ, and IL-6 in Guiding Treatment and Predicting Prognosis in the Treatment of Severe Plaque Psoriasis with Adalimumab.

Indian journal of dermatology·2025
Same author

A Case of Drug-Induced CA72-4 Pseudo-Elevation and Analysis of Its Causes.

Clinical laboratory·2025
Same author

Pseudo Elevation of CA199 Caused by Rheumatoid Factor: a Case Report and Literature Review.

Clinical laboratory·2025
Same journal

Profilin-1 as a Potential Biomarker in Acute Ischemic Stroke: Predictive Value for Large Vessel Occlusion and Mechanical Thrombectomy.

Clinical laboratory·2026
Same journal

Soluble Thrombomodulin can Predict In-Hospital Mortality of Community-Acquired Pneumonia Patients in Intensive Care Unit.

Clinical laboratory·2026
Same journal

Cytomegalovirus Seroprevalence in Morocco - a 10-Year Single-Center Study.

Clinical laboratory·2026
Same journal

Impact of Inactivated SARS-CoV-2 Vaccines on Serum Glycan Profiles and Protein N-Glycosylation.

Clinical laboratory·2026
Same journal

Albumin Concentration Associates Linearly with Unfavorable Outcomes at Three Months Post-Acute Ischemic Stroke in Koreans.

Clinical laboratory·2026
Same journal

Prognostic Value of the Blood Urea Nitrogen-to-Albumin Ratio for ICU Mortality in Cardiogenic Shock Patients: Evidence from the MIMIC-IV Database.

Clinical laboratory·2026
See all related articles

Related Experiment Video

Updated: May 15, 2025

Author Spotlight: In Vivo Assessment of Thyroid Hormone Disruption Using the THAI Mouse Model
04:14

Author Spotlight: In Vivo Assessment of Thyroid Hormone Disruption Using the THAI Mouse Model

Published on: October 6, 2023

747

Macro-TSH Interference in Thyroid Function Testing: a Case Report and Literature Review.

Hong-Gang Sun, Li-Qin He

    Clinical Laboratory
    |April 10, 2025
    PubMed
    Summary
    This summary is machine-generated.

    Macro-TSH can cause falsely elevated Thyroid-Stimulating Hormone (TSH) levels, leading to misdiagnosis. PEG6000 treatment confirmed macro-TSH in this case, preventing unnecessary thyroid treatment.

    More Related Videos

    Generation of a Mouse Spontaneous Autoimmune Thyroiditis Model
    04:39

    Generation of a Mouse Spontaneous Autoimmune Thyroiditis Model

    Published on: March 17, 2023

    1.5K
    Spontaneous Murine Model of Anaplastic Thyroid Cancer
    05:39

    Spontaneous Murine Model of Anaplastic Thyroid Cancer

    Published on: February 3, 2023

    1.6K

    Related Experiment Videos

    Last Updated: May 15, 2025

    Author Spotlight: In Vivo Assessment of Thyroid Hormone Disruption Using the THAI Mouse Model
    04:14

    Author Spotlight: In Vivo Assessment of Thyroid Hormone Disruption Using the THAI Mouse Model

    Published on: October 6, 2023

    747
    Generation of a Mouse Spontaneous Autoimmune Thyroiditis Model
    04:39

    Generation of a Mouse Spontaneous Autoimmune Thyroiditis Model

    Published on: March 17, 2023

    1.5K
    Spontaneous Murine Model of Anaplastic Thyroid Cancer
    05:39

    Spontaneous Murine Model of Anaplastic Thyroid Cancer

    Published on: February 3, 2023

    1.6K

    Area of Science:

    • Endocrinology
    • Clinical Chemistry
    • Immunology

    Background:

    • Thyroid-Stimulating Hormone (TSH) regulates thyroid hormone production via negative feedback.
    • TSH testing is vital for diagnosing and managing thyroid disorders.
    • Factors like heterophilic antibodies, thyroid hormone autoantibodies (THAb), and macro-TSH can interfere with TSH test accuracy.

    Purpose of the Study:

    • To investigate the impact of interfering factors on TSH testing.
    • To highlight the importance of identifying and excluding macro-TSH in thyroid function tests.
    • To present a case where macro-TSH led to inaccurate TSH results.

    Main Methods:

    • Utilized chemiluminescence immunoassay (CLIA) for thyroid function testing.
    • Assessed TSH, FT3, and FT4 levels in a patient with no prior thyroid disease history.
    • Employed Polyethylene Glycol 6000 (PEG6000) precipitation to identify macro-TSH interference.

    Main Results:

    • Initial TSH levels were significantly elevated (16.1751 µIU/mL) compared to the reference range.
    • Free T3, free T4, total T3, and total T4 levels were within normal limits.
    • Post-PEG6000 treatment, TSH levels normalized to 0.98 µIU/mL, confirming macro-TSH.

    Conclusions:

    • Macro-TSH can cause falsely elevated TSH, potentially leading to misdiagnosis and inappropriate treatment.
    • PEG6000 precipitation is an effective method for detecting macro-TSH.
    • Clinical correlation and exclusion of interfering factors are crucial for accurate thyroid function assessment.