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Related Concept Videos

DNA Isolation01:24

DNA Isolation

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DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
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Updated: May 15, 2025

Rapid, Safe, and Simple Manual Bedside Nucleic Acid Extraction for the Detection of Virus in Whole Blood Samples
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Acid-activated bentonite for solid-phase nucleic acid extraction from various pathogenic samples.

Eun Yeong Lee1, Minju Lee1, Myoung Gyu Kim1

  • 1Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul, 03722, Republic of Korea.

Analytica Chimica Acta
|April 10, 2025
PubMed
Summary

A novel solid-phase extraction method using amine-functionalized sulfuric acid-activated bentonite (ASAB) offers efficient and versatile nucleic acid extraction from diverse samples in under 30 minutes, significantly improving recovery rates.

Keywords:
Acid-activated bentoniteDisease diagnosticsNucleic acid isolationReversible cross-linking reactionSolid-phase extraction

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Filtration Isolation of Nucleic Acids: A Simple and Rapid DNA Extraction Method
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Filtration Isolation of Nucleic Acids: A Simple and Rapid DNA Extraction Method

Published on: August 6, 2016

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Area of Science:

  • Molecular Biology
  • Biochemistry
  • Materials Science

Background:

  • Current nucleic acid extraction methods face challenges in efficiency, complexity, and versatility.
  • Limitations hinder advancements in nucleic acid testing technologies.

Purpose of the Study:

  • To develop an innovative solid-phase extraction (SPE) method for efficient nucleic acid isolation.
  • To overcome limitations of existing nucleic acid extraction techniques.

Main Methods:

  • Sulfuric acid activation of bentonite (SAB) to enhance surface area and adsorption capacity.
  • Amine-functionalization of SAB with APDMS to create ASAB for improved nucleic acid binding.
  • Utilizing a homobifunctional imidoester (HI) reagent for reversible nucleic acid interactions with ASAB.

Main Results:

  • ASAB-based SPE system provides a universal protocol for DNA, RNA, and miRNA isolation from diverse samples in under 30 minutes.
  • Effective enrichment of low concentrations of pathogens from large sample volumes using the ASAB-HI complex.
  • Demonstrated significant improvements in DNA recovery (up to 3.95-fold) and isolation of unstable viral RNA and microRNA biomarkers compared to commercial kits.

Conclusions:

  • The ASAB-based SPE system exhibits high efficiency and versatility for nucleic acid recovery.
  • This innovative platform has the potential to revolutionize traditional SPE methods in molecular biology research.