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Efficient degradation of alternariol in food by a novel isolate, Bacillus pacificus ANSB901
Yanrong Liu1, Xinyue Zhang1, Mingxin Ma1
1State Key Laboratory of Animal Nutrition and Feeding, Poultry Nutrition and Feed Technology Innovation Team, College of Animal Science and Technology, China Agricultural University, Beijing 100193, PR China.
Abstract:
Alternariol (AOH) is a mycotoxin that contaminates food and cause severe health issues for both humans and animals. Currently there are few researches on the degradation of AOH by microorganisms and enzymes. After screening 91 strains isolated from food samples and digestive tract chyme of chickens, it was found that Bacillus pacificus ANSB901 showed the highest AOH removal ratio, and its bacterial cell lysate could reduce AOH by 94% within 12 h. B. pacificus ANSB901 can efficiently eliminate AOH in distillers dried grains with soluble (DDGS), apple juice, and moldy maize. As potential food and feed supplements, B. pacificus ANSB901 appeared to show no sign of toxicity or virulence. Three AOH degradation products (C14H12O5), (C20H20O10), and (C14H12O7) with low toxicity were observed. Based on the analysis of possible transformation pathways and whole genome sequencing of B. pacificus ANSB901, 15 protein candidates potentially involved in AOH degradation were screened for further validation. B. pacificus ANSB901 and its enzymes can serve as candidate biodegraders for AOH degradation in food and feed.
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