Evaluation of the duration of new nasal drug interferon α-2b activity in an experimental model system

To develop an adequate system for assessing the duration of IFN antiviral activity in the nasal cavity of animals and to study the antiviral activity of a new IFN drug in the form of a gel in comparison with the known spray form.

To assess the duration of the specific activity of IFN, the drugs were administered into the nasal cavity of experimental rats. One, 3, and 4 h after administration, the nasal cavities of the rats were washed out, and the antiviral activity of washed from the nasal cavity (WNC) was analyzed in vitro.

Developed an algorithm to assess the duration of antiviral activity of nasal preparations using a sensitive in vitro test system, in which the analysis of the antiviral activity of WNC made it possible to detect antiviral activity after the administration of nasal forms of IFN-a new variant in the form of a gel in comparison with the IFN spray. It was established that 1 h after the intranasal administration of IFN, the same antiviral activity and IFN concentration is: 266.8 ± 14.0 IU/ml for IFN/spray and 260.2 ± 20.9 IU/ ml for IFN/gel. Three hours after the drugs are administered, their activity decreases but remains at a sufficiently high level: 121.4 ± 5.4 IU/ml for IFN/spray and 88.3 ± 6.2 IU/ml for IFN/gel. Four hours after IFN administration, the concentration of IFN/gel was significantly greater than that of IFN/spray: 39.4 ± 4.9 IU/ml and 10.6 ± 1.0 IU/ml, respectively.

The developed system for evaluating the antiviral activity of nasal preparations allows the study of the duration of the local antiviral effect of drugs. The antiviral activity of the IFN nasal preparations persisted for 4 h after their intranasal administration: the concentration of IFN in gel form on the walls of the nasal cavity was significantly greater than that of the IFN spray.