Related Experiment Video
Updated: May 15, 2025
Scalable Isolation and Purification of Extracellular Vesicles from Escherichia coli and Other Bacteria
Published on: October 13, 2021
Assessment of Antimicrobial Potential of Lactiplantibacillus plantarum and Their Derived Extracellular Vesicles
Pooja Yadav1, Nabendu Debnath1, Praveen Kumar Mehta1
1Centre for Molecular Biology, Central University of Jammu, Rahya-Suchani (Bagla) Samba, Jammu, Jammu & Kashmir, India.
Scope:
Probiotic-derived antimicrobial substances offer a promising treatment approach with less resistance risk. This study evaluated the antimicrobial activity of 20 Lactiplantibacillus plantarum strains of human gut origin and their derived extracellular vesicles (EVs) against the pathogens Staphylococcus aureus MTCC 96 and Propionibacterium acnes MTCC 1950.
Methods And Results:
The cell-free supernatants (CFSs) of all Lactobacillus isolates were initially screened for antimicrobial potential against the selected pathogens. The CFSs of selected isolates were further concentrated and were subjected to SDS PAGE, with bands excised for analysis of antimicrobial activity. Additionally, EVs were isolated and characterized from LpJ5 and LpJ15 and evaluated their antimicrobial activity. Results indicated that out of 20 isolates, the CFS (pH 6.5) of both the isolates demonstrated the highest zones of inhibition against S. aureus (22.9 ± 1.00 mm) and P. acnes (15.66 ± 1.52 mm), respectively. The EVs derived from LpJ5 and LpJ15 showed inhibition zones of 18.5 ± 0.2 mm and 20 ± 0.3 mm against S. aureus and P. acnes.
Conclusion:
Findings from this study highlight that the EVs isolated from CFS of two potent isolates LpJ5 and LpJ15, exhibit substantial antimicrobial activity, and suggest their potential application as biotherapeutic agents against selected pathogens.
More Related Videos
06:28Isolation of Low Endotoxin Content Extracellular Vesicles Derived from Cancer Cell Lines
Published on: February 17, 2023
10:23Characterizing Mediated Extracellular Electron Transfer in Lactic Acid Bacteria with a Three-Electrode, Two-Chamber Bioelectrochemical System
Published on: August 23, 2024
Related Concept Videos
01:18Surface Membrane Barriers
The outer layer of the skin, the epidermis, is a robust barrier comprising layers of closely packed keratinized cells. This dense arrangement prevents microbes from penetrating the body. The periodic shedding of epidermal cells...
01:23Antimicrobial Proteins
Interferons
Interferons (IFNs) are proteins produced by lymphocytes, macrophages, and fibroblasts infected with viruses. While IFNs cannot prevent viruses from entering and...