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Updated: May 15, 2025
07:56
Genome Editing in Mammalian Cell Lines using CRISPR-Cas
Published on: April 11, 2019
21.4K
Exploring Advanced CRISPR Delivery Technologies for Therapeutic Genome Editing.
Neda Rostami1, Mohammad Mahmoudi Gomari2, Edris Choupani3
1Department of Chemical Engineering Arak University Arak 3848177584 Iran.
Small Science
|April 11, 2025
Summary
Engineered carriers enhance CRISPR genome editing delivery in vivo, overcoming challenges like low efficiency and off-target effects. These platforms improve stability and precision for therapeutic applications.
Area of Science:
- Biotechnology
- Molecular Biology
- Genetics
Background:
- Genetic material governs organismal structure and function.
- Genome manipulation offers potential for correcting abnormalities and introducing new traits.
- CRISPR technology provides precise genome editing capabilities.
Purpose of the Study:
- To review recent advances in CRISPR-based genome editing delivery systems.
- To explore the benefits and challenges of various engineered carrier platforms.
- To provide insights into the clinical utility and future prospects of CRISPR delivery technologies.
Main Methods:
- Review of scientific literature on CRISPR delivery systems.
- Examination of engineered carrier platforms, including nanocarriers, viral particles, and exosomes.
- Analysis of challenges such as delivery efficiency, off-target effects, and stability.
Main Results:
- Engineered carrier platforms significantly improve CRISPR delivery efficiency, stability, and precision.
- These platforms mitigate off-target effects and reduce side effects associated with genome editing.
- Various carriers, including nanocarriers, viral particles, and exosomes, show promise for in vivo applications.
Conclusions:
- Engineered carriers are crucial for advancing CRISPR genome editing for therapeutic applications.
- Further research into carrier platforms will enhance clinical utility and expand the scope of genome engineering.
- Targeted and precise delivery systems are key to overcoming current limitations of CRISPR technology.
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