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Related Concept Videos

Introduction to the Human Microbiota01:22

Introduction to the Human Microbiota

Microorganisms colonize various regions of the human body, including the mouth, nasal passages, throat, stomach, intestines, urogenital tract, and skin. The total number of microbial cells is estimated to range from 10¹³ to 10¹⁴—comparable to, or exceeding, the number of human somatic cells. This host–microbiome relationship has led to the conceptualization of humans as supraorganisms, wherein microbial communities perform vital roles in development, immunity, and disease...

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Co-culture of Living Microbiome with Microengineered Human Intestinal Villi in a Gut-on-a-Chip Microfluidic Device
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Modelling Human Gut-Microbiome Interactions in a 3D Bioelectronic Platform.

Chrysanthi-Maria Moysidou1, Douglas C van Niekerk1, Verena Stoeger1

  • 1Department of Chemical Engineering and Biotechnology University of Cambridge Cambridge CB3 0AS UK.

Small Science
|April 11, 2025
PubMed
Summary
This summary is machine-generated.

This study introduces a novel bioelectronic platform to model the human gut microbiome, revealing how bacteria and postbiotics affect gut barrier integrity. This technology offers a new way to study host-microbe interactions and screen potential therapies.

Keywords:
3D cell modelsbarrier integritybioelectronicsgut microbiomehost‐microbe interactionsorgans‐on‐chipspostbiotics

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Area of Science:

  • Biomedical Engineering
  • Microbiology
  • Gastroenterology

Background:

  • The gut microbiome significantly influences host health, impacting digestion, metabolism, and diseases like neuroinflammation.
  • Understanding host-microbiome cross-talk mechanisms is crucial but challenging due to limitations of traditional animal models.
  • Translating findings from animal models to human systems remains a significant hurdle in biomedical research.

Purpose of the Study:

  • To develop and utilize a bioelectronic platform, the e-transmembrane, for creating a 3D human intestinal model.
  • To investigate the impact of microbiota, specifically postbiotics and live bacteria, on gut barrier integrity and function.
  • To establish a physiologically relevant human model for screening therapeutic and dietary interventions.

Main Methods:

  • Establishment of a 3D human intestinal model using the e-transmembrane bioelectronic platform.
  • In-line, label-free monitoring of host-microbe cross-talk via electrochemical impedance spectroscopy over 24 hours.
  • Validation of bacterial intervention effects using microscopy and molecular biomarker quantification.

Main Results:

  • The e-transmembrane platform successfully monitored distinct host-microbe interaction patterns over time.
  • Differential effects of postbiotics and live bacteria on intestinal barrier morphology and function were observed.
  • Microscopy and biomarker analysis confirmed the distinct impacts of different bacterial interventions on host tissue.

Conclusions:

  • The e-transmembrane platform provides a powerful tool for studying host-microbiome interactions in a human-relevant context.
  • This bioengineered model facilitates the evaluation of interventions like postbiotics and probiotics on gut health.
  • The developed framework enables better screening of drug candidates and therapeutic strategies for gut-related conditions.