A Novel DNA-Based Dual-Mode Data Storage System with Interrelated Concise and Detailed Data

Area of Science:

• Biotechnology
• Materials Science
• Data Storage

Background:

• DNA offers high density and stability for archival data storage.
• Current DNA storage lacks efficient random access for frequently accessed data.

Purpose of the Study:

• To develop a novel dual-mode storage system integrating DNA archival data and nanodot active data.
• To enable efficient random access and high-capacity data storage.

Main Methods:

• A two-step process involving scanning probe lithography (SPL), DNA synthesis, and chemical immobilization.
• Data categorization and storage in distinct microregions on a substrate.
• Integration of nanodot arrays with archival DNA data and primer sequences.

Main Results:

• Demonstrated efficient random access to data files stored in different microregions.
• Showcased facile data retrieval using microscopic modalities and in situ polymerase chain reaction (PCR).
• Validated the dual-mode system's potential for excellent storage capacity.

Conclusions:

• The proposed dual-mode system effectively addresses limitations in current DNA data storage.
• Integration of nanodots and DNA facilitates efficient data access and retrieval.
• This approach advances DNA-based data storage with enhanced capacity and accessibility.