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Updated: May 14, 2025

RIBO-seq in Bacteria: a Sample Collection and Library Preparation Protocol for NGS Sequencing

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Published on: August 7, 2021

Decoding Plant Ribosomal Proteins: Multitasking Players in Cellular Games.

Dariusz Stępiński¹

¹Department of Cytophysiology, Institute of Experimental Biology, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska 141/143, 90-236 Lodz, Poland.

|April 11, 2025

Summary

Ribosomal proteins (RPs) have diverse roles beyond ribosome assembly, including specialized functions in plants and regulation of physiological processes. Their paralogs and varied expression enable unique ribosomes and protein production critical for plant development.

Keywords:

plant cellular ribosomal proteins plant development ribosomal protein paralogs ribosome heterogeneity stress response

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mRNA Interactome Capture from Plant Protoplasts

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Published on: July 28, 2017

Area of Science:

Plant molecular biology
Cellular and subcellular biology
Biochemistry

Background:

Ribosomal proteins (RPs) were historically viewed solely as ribosome components.
Emerging evidence reveals RPs engage in various translational and non-ribosomal functions.
Plants possess multiple RP paralogs, suggesting specialized roles.

Purpose of the Study:

To review the multifaceted roles of ribosomal proteins (RPs) in plants.
To explore RP functions across different cellular compartments (cytosol, mitochondria, plastids).
To discuss molecular mechanisms and cooperating factors involved in RP functions.

Main Methods:

Literature review of contemporary research on ribosomal proteins.
Analysis of RP paralog diversity and spatiotemporal expression in plants.
Examination of RP roles in ribosome biogenesis and non-ribosomal activities.

Main Results:

RPs exhibit diverse functions, including ribosome assembly, selective mRNA translation, and regulation of physiological processes.
RP paralogs contribute to ribosome heterogeneity, enabling specialized protein synthesis.
RPs function independently of ribosomes, influencing broader cellular activities.
RPs in mitochondria and plastids, besides canonical roles, impact plant development.

Conclusions:

Ribosomal proteins in plants are key players in diverse cellular functions, extending beyond protein synthesis.
Ribosome heterogeneity, driven by RP paralogs, is crucial for plant adaptation and development.
RPs acting as free molecules regulate essential physiological processes, highlighting their expanded functional repertoire.