Decoding B Cells in Autoimmune Diseases Through ScRNA + BCR-Seq: Current Knowledge and Future Directions.

Area of Science:

• Immunology and Computational Biology
• Multi-omics single-cell analysis for autoimmune disease research

Background:

• Autoimmune diseases involve complex B cell dysregulation.
• Understanding B cell immunopathology requires high-resolution techniques.
• Single-cell technologies offer unprecedented insights into cellular heterogeneity.

Purpose of the Study:

• To review the combined application of scRNA-seq and scBCR-seq in autoimmune diseases.
• To elucidate the roles of dual B-cell receptor (BCR) and B/T biphenotypic cells in autoimmunity.
• To highlight advancements and challenges in multi-omics research for precision medicine.

Main Methods:

• Systematic review of scRNA-seq and scBCR-seq principles and applications.
• Analysis of dynamic correlations between B cell transcriptomes and BCR clones.
• Focus on specific B cell subsets implicated in autoimmune pathogenesis.

Main Results:

• Demonstrated dynamic correlations between B cell transcriptomes and BCR clones.
• Identified potential roles of dual BCR B cells and B/T biphenotypic cells in disease.
• Showcased abnormal clonal expansion and autoantibody secretion in autoimmune conditions.

Conclusions:

• Combined scRNA-seq and scBCR-seq provide a multidimensional view of B cell autoimmunity.
• Dual BCR and B/T biphenotypic cells may exacerbate autoimmune diseases.
• This review aims to advance multi-omics research and precision treatment strategies for autoimmune disorders.