Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

siRNA - Small Interfering RNAs

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...

Experimental RNAi

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Global research trends, knowledge structure, and future directions in melioidosis research: a bibliometric analysis, 1990-2025.

Tropical medicine and health·2026

Same author

Functional Characterisation of NF-YCs in True Leaf Biomass Accumulation.

Plants (Basel, Switzerland)·2026

Same author

Carbon Nanostructure-Initiator Mass Spectrometry for Metabolic Biomarker Detection of Prediabetes.

Analytical chemistry·2026

Same author

Clinical outcomes of concomitant anti-PD-1 therapy and denosumab treatment in non-small cell lung cancer patients with bone metastasis: a single-center retrospective study.

Discover oncology·2026

Same author

Therapeutic applications and prospects of inhalable mesoporous silica nanoparticles in pulmonary diseases.

Nanoscale·2026

Same author

In-situ Damascus-patterning enables tunable surface electric fields for bioactive titanium implants.

Bioactive materials·2026

Search research articles

Related Experiment Video

Updated: May 14, 2025

Using Lipid Nanoparticles for the Delivery of Chemically Modified mRNA into Mammalian Cells

Using Lipid Nanoparticles for the Delivery of Chemically Modified mRNA into Mammalian Cells

Published on: June 10, 2022

CaClOH-Modified Silica Nanoparticles for mRNA Delivery.

He Xian¹, Yaping Song¹, Jingjing Qu¹

¹Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, QLD 4072, Australia.

|April 11, 2025

Summary

We developed novel spiky silica nanoparticles (SNP-CaClOH) for messenger RNA (mRNA) delivery. These nanoparticles enhance mRNA translation and cellular escape, showing superior performance in vitro and in vivo.

Keywords:

CaClOH endosomal escape mRNA delivery mRNA translation silica nanoparticles

More Related Videos

Synthesis, Functionalization, and Characterization of Fusogenic Porous Silicon Nanoparticles for Oligonucleotide Delivery

Synthesis, Functionalization, and Characterization of Fusogenic Porous Silicon Nanoparticles for Oligonucleotide Delivery

Published on: April 16, 2019

Porous Silicon Microparticles for Delivery of siRNA Therapeutics

Porous Silicon Microparticles for Delivery of siRNA Therapeutics

Published on: January 15, 2015

Related Experiment Videos

Last Updated: May 14, 2025

Using Lipid Nanoparticles for the Delivery of Chemically Modified mRNA into Mammalian Cells

Using Lipid Nanoparticles for the Delivery of Chemically Modified mRNA into Mammalian Cells

Published on: June 10, 2022

Synthesis, Functionalization, and Characterization of Fusogenic Porous Silicon Nanoparticles for Oligonucleotide Delivery

Synthesis, Functionalization, and Characterization of Fusogenic Porous Silicon Nanoparticles for Oligonucleotide Delivery

Published on: April 16, 2019

Porous Silicon Microparticles for Delivery of siRNA Therapeutics

Porous Silicon Microparticles for Delivery of siRNA Therapeutics

Published on: January 15, 2015

Area of Science:

Biomaterials Science
Nanotechnology
Molecular Biology

Background:

Messenger RNA (mRNA) technology requires efficient delivery systems for biomedical applications.
Existing delivery methods face challenges in cellular uptake and endosomal escape.

Purpose of the Study:

To synthesize and characterize a novel silica nanoparticle (SNP-CaClOH) for enhanced mRNA delivery.
To investigate the mechanisms underlying SNP-CaClOH-mediated mRNA delivery and translation.

Main Methods:

Synthesis of spiky silica nanoparticles (SNPs) followed by modification with CaClOH via thermal decomposition.
Evaluation of SNP-CaClOH as an mRNA carrier for cellular delivery in vitro and in vivo.
Analysis of endosomal escape mechanisms (proton sponge effect) and translation enhancement (mTORC1 activation).

Main Results:

SNP-CaClOH nanoparticles exhibit a unique spiky surface and CaClOH composition.
The nanoparticles effectively deliver mRNA into cells, promoting endosomal escape.
SNP-CaClOH enhances mRNA translation through Ca2+ and mTORC1 activation, demonstrating superior in vitro and in vivo performance.

Conclusions:

SNP-CaClOH represents a promising novel nanomaterial for efficient mRNA delivery.
The spiky nanotopography and CaClOH composition contribute to enhanced mRNA translation and therapeutic potential.
This development offers valuable tools for advancing mRNA technology.