Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

One-pot synthesis of highly stable CsPbBr₃@C<sub>6</sub>H<sub>7</sub>NO<sub>3</sub>S composite and its application in nitrite fluorescent sensing.

Food chemistry·2025

Same author

Effects of Lactobacillus plantarum-loaded W1/O/W2 emulsions stabilized by ultrasonically treated pea protein on DSS-induced colitis in mice.

International journal of biological macromolecules·2025

Same author

Prescribed Performance Path-Following Control for Rotor-Assisted Vehicles via an Improved Reinforcement Learning Mechanism.

IEEE transactions on neural networks and learning systems·2025

Same author

Sandwich-like electrochemical immunosensing of IL-17 based on silver nanoparticles/graphdiyne and methylene blue/UiO-66-NH<sub>2</sub>.

Mikrochimica acta·2025

Same author

A portable platform integrated with smartphones for rapid lactic acid analysis via ratiometric fluorescent imaging.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy·2025

Same author

Water-Stable MIL-MOFs Developed Through a Novel Sacrifice-Protection Strategy Inspired by Butterfly Wings' Scales for Long-Term Turn-On Fluorescence Sensing of H<sub>2</sub>S.

Small methods·2025

Same journal

High-sensitivity electrochemical aptasensor based on AuNPs/NH<sub>2</sub>-UIO-66/PEI/MCNs nanocomposite for endotoxin detection.

Mikrochimica acta·2026

Same journal

A triple-emission ratiometric fluorescence and multi-color sensing platform for the detection of 6PPD-Q based on aggregation-induced emission.

Mikrochimica acta·2026

Same journal

Non-invasive monitoring of hyperuricemia via a flexible SERS sensor with ultrahigh sensitivity.

Mikrochimica acta·2026

Same journal

Multifunctional vanadium-doped carbon dots nanozymes: preparation and applications in colorimetric sensing and tumor therapy.

Mikrochimica acta·2026

Same journal

Cationic covalent organic frameworks for simultaneous capture and high-performance enrichment of multiple angiotensins and aldosterone in human plasma.

Mikrochimica acta·2026

Same journal

Highly selective enrichment of multi-phosphopeptides by polyurea-modified macroporous adsorption resin via hydrogen-bond-mediated interaction.

Mikrochimica acta·2026

See all related articles

Search research articles

Related Experiment Video

Updated: May 14, 2025

Performing Custom MicroRNA Microarray Experiments

Performing Custom MicroRNA Microarray Experiments

Published on: October 28, 2011

CHA-based microarray with Cas12a universal readout for multiple microRNA detection.

Mingkun Liu^1,2, Lei Yan², Zhixiong Lin²

¹Department of Hepatopancreatobiliary Surgery, The First Affiliated Hospital of Fujian Medical University, Fuzhou, 350001, Fujian, China.

Mikrochimica Acta

|April 11, 2025

Summary

This summary is machine-generated.

This study presents a new ultrasensitive biosensor for detecting Hirschsprung's disease (HSCR) biomarkers. The novel method uses CRISPR-Cas12a and catalytic hairpin assembly for accurate and early diagnosis of this congenital condition.

Keywords:

Cas12a Catalytic hairpin assembly Fluorescence spectrometry Hirschsprung’s disease MicroRNAs

More Related Videos

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

Published on: April 14, 2015

A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools

A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools

Published on: August 21, 2019

Related Experiment Videos

Last Updated: May 14, 2025

Performing Custom MicroRNA Microarray Experiments

Performing Custom MicroRNA Microarray Experiments

Published on: October 28, 2011

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

Published on: April 14, 2015

A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools

A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools

Published on: August 21, 2019

Area of Science:

Biotechnology
Molecular Diagnostics
Genetics

Background:

Hirschsprung's disease (HSCR) is a congenital disorder affecting the large intestine, necessitating early diagnosis for effective management.
Accurate detection of HSCR-related microRNAs (miRNAs) is crucial for timely intervention and improved patient outcomes.

Purpose of the Study:

To develop a novel and ultrasensitive biosensing strategy for the detection of HSCR-related miRNAs.
To integrate catalytic hairpin assembly (CHA) with CRISPR-Cas12a technology for enhanced diagnostic capabilities.

Main Methods:

A two-stage biosensing approach involving array recognition and a universal readout.
Utilizing catalytic hairpin assembly (CHA) for miRNA amplification on a solid-phase microarray.
Employing CRISPR-Cas12a technology for signal amplification and detection via reporter DNA cleavage.

Main Results:

The developed biosensing strategy demonstrated high sensitivity and specificity in detecting HSCR-related miRNAs.
The method successfully validated comprehensive detection of target miRNAs associated with Hirschsprung's disease.
A spatially separated fluorescence signal was generated for reliable readout.

Conclusions:

The integrated CHA and CRISPR-Cas12a system offers a significant advancement in miRNA diagnostics for HSCR.
This novel biosensing approach holds considerable potential for broader clinical applications in disease diagnosis.
The ultrasensitive detection method provides a promising tool for early and accurate diagnosis of Hirschsprung's disease.