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DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Related Experiment Video

Updated: May 14, 2025

Performing Custom MicroRNA Microarray Experiments

Performing Custom MicroRNA Microarray Experiments

Published on: October 28, 2011

CHA-based microarray with Cas12a universal readout for multiple microRNA detection.

Mingkun Liu^1,2, Lei Yan², Zhixiong Lin²

¹Department of Hepatopancreatobiliary Surgery, The First Affiliated Hospital of Fujian Medical University, Fuzhou, 350001, Fujian, China.

Mikrochimica Acta

|April 11, 2025

Summary

This summary is machine-generated.

This study presents a new ultrasensitive biosensor for detecting Hirschsprung's disease (HSCR) biomarkers. The novel method uses CRISPR-Cas12a and catalytic hairpin assembly for accurate and early diagnosis of this congenital condition.

Keywords:

Cas12a Catalytic hairpin assembly Fluorescence spectrometry Hirschsprung’s disease MicroRNAs

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Last Updated: May 14, 2025

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Published on: August 21, 2019

Area of Science:

Biotechnology
Molecular Diagnostics
Genetics

Background:

Hirschsprung's disease (HSCR) is a congenital disorder affecting the large intestine, necessitating early diagnosis for effective management.
Accurate detection of HSCR-related microRNAs (miRNAs) is crucial for timely intervention and improved patient outcomes.

Purpose of the Study:

To develop a novel and ultrasensitive biosensing strategy for the detection of HSCR-related miRNAs.
To integrate catalytic hairpin assembly (CHA) with CRISPR-Cas12a technology for enhanced diagnostic capabilities.

Main Methods:

A two-stage biosensing approach involving array recognition and a universal readout.
Utilizing catalytic hairpin assembly (CHA) for miRNA amplification on a solid-phase microarray.
Employing CRISPR-Cas12a technology for signal amplification and detection via reporter DNA cleavage.

Main Results:

The developed biosensing strategy demonstrated high sensitivity and specificity in detecting HSCR-related miRNAs.
The method successfully validated comprehensive detection of target miRNAs associated with Hirschsprung's disease.
A spatially separated fluorescence signal was generated for reliable readout.

Conclusions:

The integrated CHA and CRISPR-Cas12a system offers a significant advancement in miRNA diagnostics for HSCR.
This novel biosensing approach holds considerable potential for broader clinical applications in disease diagnosis.
The ultrasensitive detection method provides a promising tool for early and accurate diagnosis of Hirschsprung's disease.