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Based exploration of the diagnostic value of oxidative stress-related key genes in chronic obstructive pulmonary disease

  • 0Pulmonary and Critical Care Medicine, The First People'S Hospital of Yunnan Province, the Affiliated Hospital of Kunming University of Science and Technology, 157 Jinbi Road, Xishan District, Kunming, 650032, Yunnan, China. Wangshenglanzz@163.com.
Cell Biology and Toxicology +

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April 11, 2025

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April 11, 2025

View abstract on PubMed

Summary

This summary is machine-generated.

Oxidative stress-related genes, particularly ECT2 and MMP9, show promise as biomarkers for diagnosing chronic obstructive pulmonary disease (COPD). MMP9 also plays a key role in cellular injury and macrophage polarization in COPD.

Area Of Science

  • Biomedical research
  • Genomics
  • Pulmonology

Background

  • Chronic obstructive pulmonary disease (COPD) is a leading cause of death globally.
  • Oxidative stress is a key factor in COPD pathogenesis.
  • Identifying reliable biomarkers for COPD diagnosis and treatment is crucial.

Purpose Of The Study

  • To investigate oxidative stress-related genes (OSRGs) as potential biomarkers for COPD.
  • To elucidate the molecular mechanisms underlying OSRGs in COPD development.
  • To evaluate the diagnostic and therapeutic potential of identified OSRGs.

Main Methods

  • Bioinformatics analyses including LASSO, Boruta, and SVM-RFE for hub gene identification.
  • Correlation analyses with oxidative stress biomarkers.
  • Immune cell infiltration analysis.
  • Receiver Operating Characteristic (ROC) curve analysis.
  • Validation using clinical samples, animal models, qPCR, and immunohistochemistry.

Main Results

  • Five hub genes (CA3, PPP1R15B, MAPT, MMP9, ECT2) were identified.
  • MMP9 and MAPT showed strong associations with oxidative stress biomarkers.
  • ECT2 and MMP9 demonstrated high diagnostic capability for COPD.
  • MMP9 was found to be crucial in cigarette smoke condensate (CSC)-induced oxidative stress injury, reducing apoptosis and inflammation.
  • MMP9 deprivation promoted M2 macrophage polarization.

Conclusions

  • ECT2 and MMP9 are significant oxidative stress-related genes in COPD.
  • MMP9 plays a critical role in CSC-induced cellular damage and modulates macrophage polarization.
  • These findings highlight the potential of ECT2 and MMP9 as diagnostic biomarkers and therapeutic targets for COPD.

Keywords:

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