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Published on: February 17, 2023
Ratiometric Fluorescent Sensors for Real-Time Monitoring Cellular Autophagy Levels during Diabetic Wound Healing
Fei Yang1, Xiuzhu Huo2, Xiaoyu Fu1
1Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Biomedicine Key Laboratory of Shaanxi Province, Laboratory of Tissue Engineering, the College of Life Sciences, Faculty of Life Science & Medicine, Northwest University, Xi'an 710069, Shaanxi Province, China.
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Hard-healing wounds are a serious issue faced by diabetic patients, and the cellular autophagy level is closely related to the wound healing progress. However, it is difficult to monitor the real-time autophagy levels in living organisms. In this work, we provided a new autophagy fluorescent sensor IN-NH2 based on the modification of 2-substituted quinoline, giving an excellent ability to quantify the pH fluctuation in lysosomes during autophagy. This sensor was successfully applied in the real-time monitoring of autophagy levels in a wound healing model of diabetic rats, showing potential for exploring the internal mechanism between autophagy and disease progression.
