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Raddeanin A exerts potent efficacy against non-small cell lung cancer by inhibiting cyclin-dependent kinase 6

Xian Wang1, Xiao Lin2, Yuxin Liu2

  • 1Pulmonary Division, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou Key Laboratory of Interdiscipline and Translational Medicine, Wenzhou Key Laboratory of Heart and Lung, Wenzhou, Zhejiang 325035, China; School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang 325035, China; Shanghai Fengxian District Central Hospital, No. 6600, Nanfeng Highway, Fengxian District, Shanghai 201499, China.

Translational Oncology
|April 11, 2025
PubMed
Abstract

Insights

Raddeanin A effectively inhibits non-small cell lung cancer (NSCLC) growth by halting cell cycle progression. This natural compound shows promise as a novel therapeutic agent for NSCLC treatment.

Area of Science:

  • Pharmacology
  • Oncology
  • Molecular Biology

Background:

  • Non-small cell lung cancer (NSCLC) remains a leading cause of cancer-related mortality worldwide.
  • Developing novel therapeutic agents with improved efficacy and reduced toxicity is crucial for effective NSCLC treatment.

Purpose of the Study:

  • To investigate the anti-tumor effects and underlying mechanisms of Raddeanin A in NSCLC.
  • To evaluate the efficacy of Raddeanin A both in vitro and in vivo.

Main Methods:

  • Assessed Raddeanin A's effects on NSCLC cell cycle, proliferation, migration, and invasion using flow cytometry and cell biological assays.
  • Employed network pharmacology, molecular docking, molecular dynamics, SPR, Co-IP, and immunofluorescence to identify Raddeanin A's molecular targets.
  • Evaluated in vivo anti-tumor effects using a nude mouse xenograft tumor model.

Main Results:

  • Raddeanin A significantly inhibited NSCLC cell survival, invasion, and migration, inducing G1 phase cell cycle arrest.
  • Identified Cyclin-Dependent Kinase 6 (CDK6) as a key target, with Raddeanin A demonstrating tight binding and inhibition of CDK6.
  • Confirmed Raddeanin A's inhibitory effect on NSCLC tumor growth in a preclinical in vivo model.

Conclusions:

  • Raddeanin A effectively blocks the G1 phase cell cycle by inhibiting CDK6.
  • Raddeanin A exhibits significant anti-tumor activity against NSCLC, positioning it as a potential novel therapeutic agent.
Keywords:
CDK6Cycle arrestNSCLCRaddeanin Ainhibitor

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