Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Patch Clamp

Patch Clamp

Many fundamental cell functions such as muscle contraction and nerve transmission rely on the electrical signals produced by the movement of positively and negatively charged ions across the cell membrane. One competent method to record current flowing across the whole cell or single ion channel is the patch-clamp technique.
In this method, a glass micropipette containing electrolyte solution is tightly sealed against a small portion of the cell membrane. As a result, a patch of the cell...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A Lightweight ScaleDense-Transformer Framework with Auxiliary Quantum-Inspired Bottleneck Module for Whole-Lifespan Brain Age Prediction.

Brain sciences·2026

Same author

[Clinical efficacy of acupuncture combined with estazolam on chronic insomnia].

Zhen ci yan jiu = Acupuncture research·2026

Same author

Diterpenoid and triterpenoid derivatives from <i>Euphorbia kansui</i> and their anti-inflammatory activity.

Natural product research·2026

Same author

Optimized green extraction of flavonoids from Lycium barbarum leaves using betaine-ethylene glycol NADES: Mechanisms, identification, and bioactivity.

Food chemistry·2026

Same author

Cerebrospinal fluid-contacting nucleus mediates the balance of depth during general anesthesia in mice.

Neuropharmacology·2026

Same author

Reparative "exosome-ark" for mitochondrial transplantation to reprogram macrophages and disrupt pathogenic crosstalk in pulmonary fibrosis.

Materials today. Bio·2026

Search research articles

Related Experiment Video

Updated: May 14, 2025

Measurement of Ion Concentration in the Unstirred Boundary Layer with Open Patch-Clamp Pipette: Implications in Control of Ion Channels by Fluid Flow

Measurement of Ion Concentration in the Unstirred Boundary Layer with Open Patch-Clamp Pipette: Implications in Control of Ion Channels by Fluid Flow

Published on: January 7, 2019

The viscosity response behavior of bare nanopipettes based on ionic current rectification.

Yue-Qian Zhu¹, Yu-Chen Wu¹, Xu-Fan Wang¹

¹Department of Histology and Embryology, School of Basic Medical Sciences, Xuzhou Medical University, Xuzhou, 221004, China; The Second Clinical Medical College, Xuzhou Medical University, Xuzhou, 221004, China; National Demonstration Center for Experimental Basic Medical Science Education, Xuzhou Medical University, Xuzhou, 221004, China.

|April 11, 2025

Summary

Bare nanopipettes can detect solution viscosity using ionic current rectification (ICR). This simple method offers a new way to create nanopipette viscosity probes for applications like single-cell analysis.

Keywords:

Electric double layer Electroosmotic flow Ionic current rectification Nanopipettes Viscosity

More Related Videos

Multifunctional, Micropipette-based Method for Incorporation And Stimulation of Bacterial Mechanosensitive Ion Channels in Droplet Interface Bilayers

Multifunctional, Micropipette-based Method for Incorporation And Stimulation of Bacterial Mechanosensitive Ion Channels in Droplet Interface Bilayers

Published on: November 19, 2015

A Closed-Type Wireless Nanopore Electrode for Analyzing Single Nanoparticles

A Closed-Type Wireless Nanopore Electrode for Analyzing Single Nanoparticles

Published on: March 20, 2019

Related Experiment Videos

Last Updated: May 14, 2025

Measurement of Ion Concentration in the Unstirred Boundary Layer with Open Patch-Clamp Pipette: Implications in Control of Ion Channels by Fluid Flow

Measurement of Ion Concentration in the Unstirred Boundary Layer with Open Patch-Clamp Pipette: Implications in Control of Ion Channels by Fluid Flow

Published on: January 7, 2019

Multifunctional, Micropipette-based Method for Incorporation And Stimulation of Bacterial Mechanosensitive Ion Channels in Droplet Interface Bilayers

Multifunctional, Micropipette-based Method for Incorporation And Stimulation of Bacterial Mechanosensitive Ion Channels in Droplet Interface Bilayers

Published on: November 19, 2015

A Closed-Type Wireless Nanopore Electrode for Analyzing Single Nanoparticles

A Closed-Type Wireless Nanopore Electrode for Analyzing Single Nanoparticles

Published on: March 20, 2019

Area of Science:

Nanotechnology
Analytical Chemistry
Physical Chemistry

Background:

Ionic current rectification (ICR) in nanopipettes is sensitive to solution properties.
Developing simple, effective viscosity probes is crucial for various scientific fields.

Purpose of the Study:

To investigate the ionic current rectification (ICR) response of bare nanopipettes to solution viscosity.
To establish a straightforward method for fabricating nanopipette-based viscosity probes.
To explore the potential of these probes for detecting cytoplasmic viscosity.

Main Methods:

Utilized bare nanopipettes perfused with electrolyte solutions of varying viscosities.
Investigated the ICR phenomenon under different viscosity conditions.
Optimized factors like KCl concentration, pH, and nanopipette diameter to enhance viscosity response sensitivity.

Main Results:

Achieved ICR response capabilities in bare nanopipettes to solution viscosity.
Explained the viscosity response mechanism by the interplay of electric double layer and electroosmotic flow.
Demonstrated a linear relationship between the rectification ratio and solution viscosity (0.91–40.07 mPa·s).
Showcased good reversibility and selectivity in viscosity response.

Conclusions:

Bare nanopipettes serve as effective and simple viscosity probes.
The findings provide insights for developing advanced cytoplasmic viscosity probes.
This method expands the application of nanopipettes in cellular analysis.