Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Abnormal Proliferation

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...

The Tumor Microenvironment

The Tumor Microenvironment

Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

GLI2 confers ferroptosis resistance in bladder cancer by transcriptionally upregulating PRDX1.

Apoptosis : an international journal on programmed cell death·2026

Same author

SpatioTemporal Omics Consortium: a global effort for biological discovery across species, space and time.

Nature methods·2026

Same author

Proteomic analysis reveals divergent inflammatory mechanisms of COVID-associated Guillain-Barré syndrome.

Journal of molecular medicine (Berlin, Germany)·2026

Same author

Probing the limits of genetic recoding using multi-omics-guided evolution.

Nature communications·2026

Same author

EasySCP unveils extensive liver zonation at single-cell proteomics resolution.

Nature communications·2026

Same author

Advances in neuroprostheses: interfaces, materials, and applications.

Nano convergence·2026

Search research articles

Related Experiment Video

Updated: May 14, 2025

Targeting Drugs to Larval Zebrafish Macrophages by Injecting Drug-Loaded Liposomes

Targeting Drugs to Larval Zebrafish Macrophages by Injecting Drug-Loaded Liposomes

Published on: February 18, 2020

ZEB2 is a master switch controlling the tumor-associated macrophage program.

Fadi Sheban¹, Truong San Phan¹, Ken Xie¹

¹Department of Systems Immunology, Weizmann Institute of Science, Rehovot 7610001, Israel.

|April 11, 2025

Summary

Tumor-associated macrophages (TAMs) suppress immune responses. Our study reveals Zeb2 as a master regulator of TAMs, reprogramming them to enhance anti-tumor immunity and improve patient prognosis.

Keywords:

CRISPR TAM reprogramming Zeb2 cancer immunology cancer immunotherapy deep generative modeling regulatory network single cell genomics systems immunolgy tumor-associated macrophage

More Related Videos

In Vitro Assay to Study Tumor-macrophage Interaction

In Vitro Assay to Study Tumor-macrophage Interaction

Published on: August 1, 2019

Macrophage Differentiation and Polarization into an M2-Like Phenotype using a Human Monocyte-Like THP-1 Leukemia Cell Line

Macrophage Differentiation and Polarization into an M2-Like Phenotype using a Human Monocyte-Like THP-1 Leukemia Cell Line

Published on: August 2, 2021

Related Experiment Videos

Last Updated: May 14, 2025

Targeting Drugs to Larval Zebrafish Macrophages by Injecting Drug-Loaded Liposomes

Targeting Drugs to Larval Zebrafish Macrophages by Injecting Drug-Loaded Liposomes

Published on: February 18, 2020

In Vitro Assay to Study Tumor-macrophage Interaction

In Vitro Assay to Study Tumor-macrophage Interaction

Published on: August 1, 2019

Macrophage Differentiation and Polarization into an M2-Like Phenotype using a Human Monocyte-Like THP-1 Leukemia Cell Line

Macrophage Differentiation and Polarization into an M2-Like Phenotype using a Human Monocyte-Like THP-1 Leukemia Cell Line

Published on: August 2, 2021

Area of Science:

Immunology
Cancer Biology
Genomics

Background:

Tumor-associated macrophages (TAMs) are crucial for tumor immune evasion.
The regulatory mechanisms governing TAM functions remain incompletely understood.

Purpose of the Study:

To elucidate the regulatory circuits of TAMs.
To identify key regulators of TAM function and therapeutic targets.

Main Methods:

Integration of human tumor single-cell RNA sequencing (scRNA-seq) data with CRISPR screening.
Utilized a deep generative model to construct a gene perturbation network.
Investigated Zeb2's role through genetic ablation and in vivo targeting.

Main Results:

Identified Zeb2 as the master regulator of TAM programs, controlling immune suppression and antigen presentation.
Demonstrated that Zeb2 ablation reprograms TAMs at multiple molecular levels.
Found Zeb2 expression correlates with poor prognosis in human tumors.
Showed Zeb2 targeting in vivo reprograms TAMs and enhances systemic T cell responses for tumor clearance.

Conclusions:

Zeb2 acts as a master switch for TAM regulatory circuits.
Targeting Zeb2 holds significant therapeutic potential for reprogramming TAMs and improving anti-tumor immunity.