The protocol presented in this study illustrates the effectiveness of the cAMP Difference Detector In Situ in measuring cAMP through two methods. One method utilizes a 96-well plate reading spectrophotometer with HEK-293 cells. The other method demonstrates individual HASM cells under a fluorescent...

Source: Laboratories of Judith Danovitch and Nicholaus Noles—University of Louisville
How does a person learn about the world around them? One way is through direct observation and exploration. However, not every piece of information can be observed firsthand. Instead, a person must often rely on other people as information sources. This is particularly true for children who have so many questions about the world around them, yet have limited means of accessing the answers. Thus, children...

Source: Laboratories of Judith Danovitch and Nicholaus Noles—University of Louisville
Imagine a young child hears an adult say, “I want to watch the news,” then watches the adult press a button on the remote control. A moment later, the television screen turns on. The next day, the child wants to turn on the television screen to watch cartoons. How does the child know what to do? Is it enough to say, “I want to watch cartoons,” or is pushing the button on the...

This curriculum outlines how to execute a simulation-based boot camp to teach providers how to manage mechanically ventilated...

Working memory predicts a significant amount of variance for a variety of cognitive tasks, including speaking, reading, and writing. However, few tools are available to assess working memory in children. We present an innovative, computer-based battery that comprehensively assesses different components of working memory in school-age...

Cyclic Adenosine Monophosphate (cAMP) is an essential second messenger that activates protein kinase A (PKA) and regulates various biological processes. A single epinephrine molecule binds to GPCR and activates several heterotrimeric G proteins, each stimulating multiple adenylyl cyclase, amplifying the signal, and synthesizing large numbers of cAMP molecules. Small changes in cAMP concentration affect PKA activity. The binding of four cAMP molecules induces a conformational change in PKA,...