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Identification of a Highly Conserved Region Critical for the Functionality of the Cp190 Protein in Drosophila

L S Melnikova¹, K I Balagurov², P G Georgiev²

¹Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia. lsm73@mail.ru.

Doklady. Biochemistry and Biophysics

|April 11, 2025

Abstract:

The CP190 protein binds to both housekeeping gene promoters and insulator/boundary elements and plays a critical role in their activity. The aim of this work was to study the effect of deletions in highly conserved regions of the CP190 protein on its functionality. It was shown that deletion of the sequence from 664 to 700 aa leads to a lethal phenotype. Thus, a new region was identified in CP190 that plays an important role in the interaction of the CP190 protein with as yet unidentified partner proteins, stabilization of protein complexes formed by CP190, and their recruitment to chromatin.

Keywords:

CP190 architectural C2H2 proteins insulator promoter protein–protein interactions transcription regulation

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