HoloStream: a GPU-powered high-speed user interface for holographic microscopy imaging

Johan Morales , Sofia Obando-Vásquez , Ana Doblas

Optics Express +

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April 12, 2025

View abstract on PubMed

Summary

HoloStream is a new GPU-powered interface for holographic microscopy. It enables rapid reconstruction of quantitative phase images, enhancing live microscopy applications with high-speed processing and user-friendly features.

Area Of Science

Microscopy
Optical Imaging
Computational Imaging

Background

Digital holographic microscopy (DHM) offers quantitative phase imaging capabilities.
High-speed processing is crucial for real-time applications in DHM.
Existing DHM systems often face limitations in reconstruction speed and user interface flexibility.

Purpose Of The Study

To present HoloStream, a GPU-powered user interface for high-speed holographic microscopy.
To enable rapid reconstruction of quantitative phase images for off-axis DHM systems.
To enhance the usability and performance of DHM for live imaging applications.

Main Methods

Development of a GPU-powered interface (HoloStream) using PyCUDA for acceleration.
Optimization of computational procedures for quantitative phase reconstruction.
Implementation of real-time phase compensation by upgrading the semi-heuristic phase compensation (SHPC) algorithm.

Main Results

HoloStream reconstructs 1280x960 pixel phase maps at 11 frames per second (FPS) for live visualization.
The platform achieves 90 FPS for pre-recorded holographic videos.
Demonstrated capability for fast and accurate phase imaging in live microscopy experiments.

Conclusions

HoloStream significantly advances DHM technology by integrating high-speed processing with an intuitive user interface.
The platform is suitable for a wide range of DHM imaging applications requiring real-time performance.
HoloStream facilitates faster and more accurate quantitative phase imaging in live microscopy.

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