Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

9.2K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
9.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

HoloBio: A holographic microscopy tool for quantitative biological analysis.

PLoS computational biology·2026
Same author

Classification of microplastics in field-collected stream water using a submersible single-shot lensless polarimetric holographic system.

Optics express·2026
Same author

3D Image Acquisition and Display: Technology, Perception and Applications 2025: introduction.

Applied optics·2026
Same author

Feature issue introduction: 3D image acquisition and display: technology, perception and applications.

Optics express·2026
Same author

Deoxygenation-free digital holographic microscopy discriminates sickle-cell disease in a piloted Colombian cohort via phase and shape metrics.

Optics express·2026
Same author

Generalized computational framework for phase image reconstruction in structured illumination digital holographic microscopy.

Optics express·2025

Related Experiment Video

Updated: May 5, 2026

Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects
10:16

Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects

Published on: February 9, 2014

11.5K

HoloStream: a GPU-powered high-speed user interface for holographic microscopy imaging.

Johan Morales, Sofia Obando-Vásquez, Ana Doblas

    Optics Express
    |April 12, 2025
    PubMed
    Summary
    This summary is machine-generated.

    HoloStream is a new GPU-powered interface for holographic microscopy. It enables rapid reconstruction of quantitative phase images, enhancing live microscopy applications with high-speed processing and user-friendly features.

    More Related Videos

    Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization
    10:28

    Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

    Published on: July 5, 2016

    9.5K
    Evaluation and Manipulation of Neural Activity Using Two-Photon Holographic Microscopy
    10:09

    Evaluation and Manipulation of Neural Activity Using Two-Photon Holographic Microscopy

    Published on: September 16, 2022

    4.0K

    Related Experiment Videos

    Last Updated: May 5, 2026

    Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects
    10:16

    Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects

    Published on: February 9, 2014

    11.5K
    Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization
    10:28

    Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

    Published on: July 5, 2016

    9.5K
    Evaluation and Manipulation of Neural Activity Using Two-Photon Holographic Microscopy
    10:09

    Evaluation and Manipulation of Neural Activity Using Two-Photon Holographic Microscopy

    Published on: September 16, 2022

    4.0K

    Area of Science:

    • Microscopy
    • Optical Imaging
    • Computational Imaging

    Background:

    • Digital holographic microscopy (DHM) offers quantitative phase imaging capabilities.
    • High-speed processing is crucial for real-time applications in DHM.
    • Existing DHM systems often face limitations in reconstruction speed and user interface flexibility.

    Purpose of the Study:

    • To present HoloStream, a GPU-powered user interface for high-speed holographic microscopy.
    • To enable rapid reconstruction of quantitative phase images for off-axis DHM systems.
    • To enhance the usability and performance of DHM for live imaging applications.

    Main Methods:

    • Development of a GPU-powered interface (HoloStream) using PyCUDA for acceleration.
    • Optimization of computational procedures for quantitative phase reconstruction.
    • Implementation of real-time phase compensation by upgrading the semi-heuristic phase compensation (SHPC) algorithm.

    Main Results:

    • HoloStream reconstructs 1280x960 pixel phase maps at 11 frames per second (FPS) for live visualization.
    • The platform achieves 90 FPS for pre-recorded holographic videos.
    • Demonstrated capability for fast and accurate phase imaging in live microscopy experiments.

    Conclusions:

    • HoloStream significantly advances DHM technology by integrating high-speed processing with an intuitive user interface.
    • The platform is suitable for a wide range of DHM imaging applications requiring real-time performance.
    • HoloStream facilitates faster and more accurate quantitative phase imaging in live microscopy.