HoloStream: a GPU-powered high-speed user interface for holographic microscopy imaging

This paper presents HoloStream, a GPU-powered high-speed user interface designed for holographic microscopy imaging. The platform reconstructs quantitative phase images rapidly for off-axis digital holographic microscopy (DHM) systems operating in the telecentric regime. By optimizing critical computational procedures, the HoloStream app reconstructs phase maps of 1280 × 960 pixels up to 11 frames per second (FPS) in live visualization and 90 fps for pre-recorded holographic videos. This real-time phase compensation is performed by upgrading the semi-heuristic phase compensation (SHPC) algorithm through GPU acceleration via PyCUDA. The interface also offers a user-friendly experience with flexible control over acquisition modes, visualization formats, and 3D tracking functionalities. Experimental validation demonstrates the platform's capability to achieve fast and accurate phase imaging for live microscopy applications. The HoloStream app advances DHM technology by combining high-speed processing with ease of use, making it suitable for a wide range of DHM imaging applications.