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Light-Driven Deracemization by a Designed Photoenzyme.

Min Li1, Yan Zhang2, Kai Fu1

  • 1Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, P. R. China.

Journal of the American Chemical Society
|April 12, 2025
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Summary
This summary is machine-generated.

Researchers engineered a novel artificial photoenzyme using genetic code expansion to achieve catalytic deracemization of cyclopropane. This breakthrough enables new biocatalysis for challenging chemical transformations.

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Area of Science:

  • Biocatalysis
  • Protein Engineering
  • Synthetic Biology

Background:

  • Enzymes with abiological abilities offer new biocatalytic routes.
  • Traditional biocatalysis struggles with thermodynamically unfavorable reactions like cyclopropane deracemization.

Purpose of the Study:

  • To repurpose a novel protein scaffold (CTB10) as an artificial photoenzyme.
  • To enable the catalytic deracemization of cyclopropane using engineered biocatalysis.

Main Methods:

  • Genetic code expansion was used to create the artificial photoenzyme.
  • Directed evolution was employed for structural optimization.
  • X-ray crystallography was used to determine the enzyme-substrate complex structure.

Main Results:

  • The engineered CTB10-based photoenzyme achieved catalytic deracemization of cyclopropane.
  • Broad substrate scope and high enantioselectivities were obtained after optimization.
  • Structural analysis revealed a sculpted chiral cavity facilitating the reaction.

Conclusions:

  • This study demonstrates the potential of engineered photoenzymes for challenging deracemization reactions.
  • The developed artificial photoenzyme expands the scope of biocatalysis beyond natural enzymes.