Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

6.8K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
6.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Actin scaffolds as organizers of presynaptic function, assembly and plasticity across species.

Biological chemistry·2026
Same author

Local autophagy impairment triggers brain-wide presynaptic remodeling and resilience.

The EMBO journal·2026
Same author

Active zone plasticity couples sleep need to presynaptic hypophosphorylation.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Semi-automatic 3D-quantification of in-vivo synapse formation.

BMC bioinformatics·2026
Same author

How to control synaptic autophagy from the neuronal soma.

The EMBO journal·2025
Same author

Monoamine-induced diacylglycerol signaling rapidly accumulates Unc13 in nanoclusters for fast presynaptic potentiation.

Proceedings of the National Academy of Sciences of the United States of America·2025

Related Experiment Video

Updated: May 14, 2025

Visualization of the Immunological Synapse by Dual Color Time-gated Stimulated Emission Depletion STED Nanoscopy
10:00

Visualization of the Immunological Synapse by Dual Color Time-gated Stimulated Emission Depletion STED Nanoscopy

Published on: March 24, 2014

78.0K

Imaging Synapse Ultrastructure and Organization with STED Microscopy.

Marta Maglione1,2,3, Stephan J Sigrist4,5

  • 1Institute for Biology, Freie Universität Berlin, Berlin, Germany.

Methods in Molecular Biology (Clifton, N.J.)
|April 12, 2025
PubMed
Summary

Super-resolution microscopy resolves nanoscale protein organization in neuronal synapses. This study details a method using time-gated STED microscopy to map active zone proteins relative to calcium channels in situ.

Keywords:
Active zoneBrain slicesCav2.1Mossy fiberRIM1Super-resolutionSynapsegSTED

More Related Videos

Author Spotlight: Decoding Mitochondrial Aging
08:48

Author Spotlight: Decoding Mitochondrial Aging

Published on: June 30, 2023

3.4K
Visualizing the Actin and Microtubule Cytoskeletons at the B-cell Immune Synapse Using Stimulated Emission Depletion STED Microscopy
11:00

Visualizing the Actin and Microtubule Cytoskeletons at the B-cell Immune Synapse Using Stimulated Emission Depletion STED Microscopy

Published on: April 9, 2018

13.6K

Related Experiment Videos

Last Updated: May 14, 2025

Visualization of the Immunological Synapse by Dual Color Time-gated Stimulated Emission Depletion STED Nanoscopy
10:00

Visualization of the Immunological Synapse by Dual Color Time-gated Stimulated Emission Depletion STED Nanoscopy

Published on: March 24, 2014

78.0K
Author Spotlight: Decoding Mitochondrial Aging
08:48

Author Spotlight: Decoding Mitochondrial Aging

Published on: June 30, 2023

3.4K
Visualizing the Actin and Microtubule Cytoskeletons at the B-cell Immune Synapse Using Stimulated Emission Depletion STED Microscopy
11:00

Visualizing the Actin and Microtubule Cytoskeletons at the B-cell Immune Synapse Using Stimulated Emission Depletion STED Microscopy

Published on: April 9, 2018

13.6K

Area of Science:

  • Neurobiology
  • Cellular Biology
  • Microscopy

Background:

  • Understanding protein function requires knowing their cellular localization and interactions.
  • Synapses, crucial in neurobiology, possess structures often below conventional light microscopy resolution.
  • Super-resolution light microscopy (SRLM) overcomes diffraction limits, enabling visualization of nanoscale subsynaptic structures.

Purpose of the Study:

  • To present a method for precisely localizing active zone scaffolding proteins in relation to voltage-gated calcium channels.
  • To investigate the nanoscale organization within the presynaptic active zone in situ.

Main Methods:

  • Utilizing time-gated stimulated emission depletion (gSTED) microscopy, a type of SRLM.
  • Applying the method to image the presynaptic active zone in situ.
  • Focusing on the spatial relationship between active zone scaffolding proteins and voltage-gated calcium channels.

Main Results:

  • The study outlines a method enabling high-resolution imaging of the presynaptic active zone.
  • The technique allows for the determination of the relative localization of key active zone proteins and calcium channels.
  • This provides insights into the nanoscale architecture of synaptic transmission sites.

Conclusions:

  • Time-gated STED microscopy is a powerful tool for dissecting the molecular organization of synaptic active zones.
  • The developed method facilitates a deeper understanding of synaptic function by revealing protein positioning.
  • This research contributes to advancing neurobiology through high-resolution imaging techniques.