Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Rabies

Rabies

Rabies is a lethal zoonotic disease caused by a single-stranded, negative-sense RNA virus of the Lyssavirus genus, within the family Rhabdoviridae. Its primary mode of transmission to humans is through bites or saliva-contaminated scratches from infected mammals such as dogs, bats, raccoons, or foxes. Transmission can also occur if infectious saliva contacts abraded skin or intact mucous membranes, including the conjunctiva.Viral Entry and Early ReplicationOnce introduced at the bite or scratch...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Transcriptomics reveals pallial and subpallial subdivisions of the mouse medial amygdala.

Brain structure & function·2026

Same author

Transcriptomic Analysis Corroborates the New Radial Model of the Mouse Pallial Amygdala.

Biomolecules·2025

Same author

Adolescent seizure impacts oligodendrocyte maturation, neuronal-glial circuit Formation, and myelination in the mammalian forebrain.

Neuroscience·2024

Same author

Shh from mossy cells contributes to preventing NSC pool depletion after seizure-induced neurogenesis and in aging.

eLife·2023

Same author

Neuronal Activity Changes the Number of Neurons That Are Synaptically Connected to OPCs.

eNeuro·2023

Same author

Shh from mossy cells contributes to preventing NSC pool depletion after seizure-induced neurogenesis and in aging.

bioRxiv : the preprint server for biology·2023

Search research articles

Related Experiment Video

Updated: Jul 2, 2026

Viral Tracing of Genetically Defined Neural Circuitry

Viral Tracing of Genetically Defined Neural Circuitry

Published on: October 17, 2012

Monosynaptic Tracing in Developing Circuits Using Modified Rabies Virus.

Laura Cocas^1,2, Gloria Fernandez³

¹Department of Biology, Santa Clara University, Santa Clara, CA, USA. lcocas@scu.edu.

Methods in Molecular Biology (Clifton, N.J.)

|April 12, 2025

Summary

Researchers developed a new rabies virus system for mapping neuronal connections in vivo. This method allows precise analysis of presynaptic inputs to specific neuron types in both developing and mature brains.

Keywords:

Monosynaptic circuit tracing Neuronal circuit development Rabies virus Stereotaxic surgery Viral amplification

More Related Videos

Transsynaptic Tracing from Peripheral Targets with Pseudorabies Virus Followed by Cholera Toxin and Biotinylated Dextran Amines Double Labeling

Transsynaptic Tracing from Peripheral Targets with Pseudorabies Virus Followed by Cholera Toxin and Biotinylated Dextran Amines Double Labeling

Published on: September 14, 2015

Large-scale Reconstructions and Independent, Unbiased Clustering Based on Morphological Metrics to Classify Neurons in Selective Populations

Large-scale Reconstructions and Independent, Unbiased Clustering Based on Morphological Metrics to Classify Neurons in Selective Populations

Published on: February 15, 2017

Related Experiment Videos

Last Updated: Jul 2, 2026

Viral Tracing of Genetically Defined Neural Circuitry

Viral Tracing of Genetically Defined Neural Circuitry

Published on: October 17, 2012

Transsynaptic Tracing from Peripheral Targets with Pseudorabies Virus Followed by Cholera Toxin and Biotinylated Dextran Amines Double Labeling

Transsynaptic Tracing from Peripheral Targets with Pseudorabies Virus Followed by Cholera Toxin and Biotinylated Dextran Amines Double Labeling

Published on: September 14, 2015

Large-scale Reconstructions and Independent, Unbiased Clustering Based on Morphological Metrics to Classify Neurons in Selective Populations

Large-scale Reconstructions and Independent, Unbiased Clustering Based on Morphological Metrics to Classify Neurons in Selective Populations

Published on: February 15, 2017

Area of Science:

Neuroscience
Molecular Biology
Genetics

Background:

Analyzing neuronal connectivity is crucial for understanding brain function.
Traditional methods for mapping neural circuits can be labor-intensive and lack specificity.
Rabies virus has shown potential for tracing neural circuits due to its retrograde properties.

Purpose of the Study:

To develop and describe a novel method for analyzing retrograde monosynaptic connections in vivo.
To enable subtype-specific targeting of neuronal populations for circuit analysis.
To provide a comprehensive protocol for researchers interested in mapping neuronal inputs.

Main Methods:

Utilizing an attenuated rabies virus engineered to express fluorescent proteins.
Implementing a Cre-loxP-based system for genetic targeting of specific cell subtypes.
Employing stereotaxic procedures for precise viral vector delivery in vivo.
Amplifying deletion mutant, pseudotyped rabies virus for enhanced tracing.

Main Results:

The described rabies virus system allows for the visualization of retrograde (presynaptic) monosynaptic connections.
The combination with Cre-loxP enables precise targeting of neuronal subtypes for input analysis.
The method is applicable to studying neural circuits in both developing and mature brains.
Detailed protocols for virus amplification, cell targeting, and stereotaxic injection are provided.

Conclusions:

This advanced rabies virus tracing technique offers a powerful tool for in vivo neural circuit analysis.
The method facilitates the study of neuronal input onto specific neuron classes with high precision.
The described approach enhances the ability to investigate neural connectivity across different brain developmental stages.