Temporal transcriptome analysis reveals the two-phase action of florigens in rice flowering

Two florigen genes, Hd3a and RFT1, are essential for the floral transition in rice. To elucidate the early steps of the transcriptional dynamics during rice floral induction, we compared a set of temporal transcriptome data of SAM (shoot apical meristem)-region samples between the wild-type and a non-flowering line of the hd3a rft1 double mutant during a short-day (SD) treatment after growing under long-day conditions for 42 days, and identified 6,978 DEGs (differentially expressed genes). As expected, FUL-like MADS-box genes were induced just after 4 days SD treatment; meanwhile, SEP-type and AGL-type MADS-box genes were induced after 9 days of SD treatment. We here newly revealed that majority of rhythmic genes including major circadian clock genes were not affected by the florigen genes, implying normal circadian clock phasing at the SAM regions regardless of floral transitions. We found that around two thousands of genes were repressed by Hd3a and RFT1 genes at the SAM regions before the SD treatments and become derepressed and similar to WT expression levels in the double mutants according to the SD treatments. These clearly imply two distinct actions of florigen genes: one for repression of some developmental key genes during vegetative growth possibly by very low level of florigen expression and the other for floral induction by relatively high florigen expressions upon short-day inductions. This repression by low levels of florigens may serve as a maintenance system for vegetative growth before floral induction, which implies a novel role for florigen genes in rice.