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First-in-kind 3D bioprinted human skin model using recombinant human collagen.

Hemanth Gudapati1, Rafaela Mayumi Simoes Torigoe2, Aydin Tahmasebifar1

  • 1Department of Dermatology, Mayo Clinic, 200 1st SW Street, Rochester, MN, 55905, USA.

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Summary
This summary is machine-generated.

Researchers developed a novel 3D bioprinted human skin model using plant-derived collagen. This innovative model offers a promising alternative to animal testing for drug development and regenerative medicine.

Keywords:
3D Bioprinting3D humanized skin modelPreclinical skin modelRecombinant human collagen

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Area of Science:

  • Biotechnology
  • Regenerative Medicine
  • Dermatology

Background:

  • Lack of reliable in-vitro human skin models hinders disease modeling and therapeutic screening.
  • 3D bioprinting technology offers a potential solution for fabricating complex biological structures.
  • Animal testing for preclinical research faces ethical and translational limitations.

Purpose of the Study:

  • To develop a fully humanized 3D bioprinted skin model as an alternative to animal testing.
  • To evaluate the structural and functional resemblance of the bioprinted skin to native human skin.
  • To explore the potential of plant-derived recombinant human collagen in skin bioprinting.

Main Methods:

  • Fabrication of a 3D bioprinted skin model using plant-derived recombinant human collagen.
  • Incorporation of human skin fibroblasts, melanocytes, and keratinocytes.
  • Analysis of skin model characteristics, including protein expression, melanin content, and epidermal stratification.

Main Results:

  • The 3D bioprinted skin model expressed key markers (involucrin, cytokeratin 14) and contained melanin granules.
  • The model exhibited epidermal stratification, indicating skin maturation, though keratinocyte morphology differed slightly from native skin.
  • The model currently lacks skin appendages like hair follicles and sweat glands due to technological limitations.

Conclusions:

  • 3D bioprinting with plant-derived recombinant human collagen can create a viable human skin model for preclinical research.
  • This technology offers advantages such as high-throughput production, personalization, and reduced animal testing.
  • Future advancements in bioprinting may overcome current limitations, expanding applications in regenerative medicine.