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Visualization and Quantification of NFAT1 Translocation in T Cells
Franziska Möckl1, Dejan Kovacevic1,2,3,4, René Werner2,3,4
1The Calcium Signalling Group, Department of Biochemistry and Molecular Cell Biology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
Abstract:
NFAT1 translocation to the nucleus and consequent IL-2 production in T cells can be used as a downstream read-out for initial T cell activation via the T cell receptor complex. Here, we describe a protocol for the detection of NFAT1 translocation using anti-CD3/anti-CD28 coated beads as a stimulus of primary murine CD4+ T cells by immunohistochemistry. Further, an open-source Python code is described to determine NFAT1 translocation.
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