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Updated: May 23, 2026

Nuclei Isolation from Whole Tissue using a Detergent and Enzyme-Free Method
Published on: June 24, 2020
Protocol for isolating nuclei from frozen fish heart tissue for single-cell genomic assays
Chaoguang Wei1, Ruonan Jia2, Adelino V M Canário3
1Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China; International Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai Ocean University, Shanghai 201306, China.
None:
Unbiased isolation of intact single nuclei from complex tissues, such as cryopreserved hearts, for massively parallel single-cell genomic assays is particularly challenging. Here, we present a protocol for isolating high-quality nuclei from the heart tissue of Trematomus bernacchii, an Antarctic fish species with notoriously challenging tissue for nuclear extraction. We describe steps for pulverizing frozen heart tissue, library construction, and sequencing. This protocol can provide a valuable reference for isolating nuclei from other cryopreserved marine fish tissues.
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