Related Experiment Video
Updated: May 17, 2025

Murine Excisional Wound Healing Model and Histological Morphometric Wound Analysis
Published on: August 21, 2020
Repurposing of activating transcription factor 3 (ATF3) activator molecules with potential wound-healing effects
Yolanda M Jacobo-Delgado1, Valentin Trujillo-Paez1, Alan Santos-Mena1
1Zacatecas Biomedical Research Unit, Mexican Social Security Institute. Interior de la Alameda #45 Colonia Centro ZIP code 98000, Zacatecas, Mexico.
Background:
Wound healing is a complex and regulated process that involves the coordinated action of key signaling pathways. Activating transcription factor 3 (ATF3) is a stress-inducible protein that has recently emerged as a critical modulator of cellular responses to injury, including those involved in wound healing.
Aim:
The aim of this study was to explore the repurposing of existing pharmacological agents to activate ATF3 and evaluate their potential to enhance wound healing factors.
Methods:
We selected three compounds: retin-A, furosemide, and acrivastine based on their ability to modulate ATF3 expression and assessed their effects on wound healing processes in primary cell cultures. We evaluated wound healing-related genes such as LL-37, HBD-2, HBD-3, and VEGFA by qPCR, and a wound healing scratch assay using keratinocytes was conducted to evaluate cell migration.
Results:
Interestingly, retin-A induced the expression of key wound healing-related genes, including HBD-2, HBD-3, LL-37, and VEGF. Also, retin-A was the only compound showing wound healing effects, while furosemide and acrivastine did not exhibit any noticeable activity.
Conclusion:
Our research highlights the potential of retin-A as therapeutic agents to improve wound healing, particularly in chronic wound models.
More Related Videos
09:06Assessment of Acute Wound Healing using the Dorsal Subcutaneous Polyvinyl Alcohol Sponge Implantation and Excisional Tail Skin Wound Models.
Published on: March 25, 2020
09:23Optimizing Extracellular Vesicle Delivery Using a Core-Sheath 3D-Bioprinted Scaffold for Chronic Wound Management
Published on: February 28, 2025
Related Concept Videos
Clinical Applications of Epidermal Stem Cells
Eukaryotic Transcription Activators
The binding domains are capable of recognizing and interacting with regulatory sequences on the DNA. These...
TGF - β Signaling Pathway
Regulation of Angiogenesis and Blood Supply