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HPLC method for novel cannabinoid separation with electrochemical detection on boron-doped diamond electrode
Zeynab Belbasi1, Jan Petr1, Jan Hrbac2
1Palacky University, Faculty of Science, Department of Analytical Chemistry, 17. listopadu 12, Olomouc 771 46, Czech Republic.
Journal of Pharmaceutical and Biomedical Analysis
|April 12, 2025
Summary
A new, eco-friendly method uses high-performance liquid chromatography and a boron-doped diamond electrode to detect cannabinoids like HHC. This sensitive technique offers rapid analysis with nanomolar detection limits for various cannabinoid compounds.
Area of Science:
- Analytical Chemistry
- Electrochemistry
- Chromatography
Background:
- Cannabinoid analysis is crucial for quality control and research.
- Existing methods may have limitations in sensitivity, speed, or environmental impact.
- Novel electrochemical detection offers potential for improved cannabinoid determination.
Purpose of the Study:
- To develop and validate an environmentally friendly HPLC-ECD method for analyzing key cannabinoids.
- To utilize a boron-doped diamond electrode for enhanced detection capabilities.
- To achieve sensitive and rapid quantification of cannabinoids in commercial products.
Main Methods:
- High-performance liquid chromatography (HPLC) with electrochemical detection (ECD).
- Boron-doped diamond (BDD) working electrode operated at +1400 mV (vs. Pd/H₂).
- Reversed-phase Gemini C18 column with acetonitrile/phosphate buffer mobile phase (step-gradient).
Main Results:
- Optimized method achieved cannabinoid separation in under 25 minutes.
- Limits of detection (LOD) ranged from 6.1 to 666 nM.
- Linear response observed up to 10 μM with good linearity, precision, and sensitivity.
- Successful application to HHC jelly bears and distillate samples.
Conclusions:
- The developed HPLC-ECD method is sensitive, precise, and environmentally friendly.
- BDD electrode provides superior performance for cannabinoid analysis.
- The method is suitable for the quantification of cannabinoids in complex matrices.
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