Microbial polysaccharides biosynthesis and their regulatory strategies

Microbial polysaccharides hold significant potential for various applications, including food, cosmetics, petroleum, and pharmaceuticals. A deeper understanding of their biosynthetic pathways and regulatory strategies is crucial for enhancing production efficiency and reducing associated costs. To summarize synthetic biological modification strategies for microbial polysaccharides from a hierarchical perspective, this review classifies these polymers into three categories based on the depths of carried out research regarding their biosynthetic pathways and regulatory strategies, i.e., (1) microbial polysaccharides with well-elucidated biosynthetic pathways, (2) microbial polysaccharides with well-elucidated precursor sugar biosynthetic pathways but synthase-encoding genes incompletely understood, and (3) those whose biosynthesis depends on a single synthetic enzyme. We systematically summarize the biosynthetic pathways of these three categories and provide insights into yield-improvement strategies. This review aims to serve as a valuable reference for metabolic regulation of microbial polysaccharides and to facilitate future advances in their production.