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Osthole improves erectile function by increasing endogenous H2S production.

Elif Alan-Albayrak1, Estéfano Pinilla2, Simon Comerma-Steffensen3

  • 1Department of Pharmacology, Faculty of Pharmacy, Ege University, Izmir, Türkiye; Department of Biomedicine, Pulmonary and Cardiovascular Pharmacology, Aarhus University, Aarhus, Denmark.

European Journal of Pharmacology
|April 12, 2025
PubMed
Summary

Osthole enhances erectile function by increasing hydrogen sulfide (H2S) production, offering a potential new treatment for erectile dysfunction (ED). This study reveals H2S

Keywords:
Corpus cavernosumErectile functionHydrogen sulfideMousePudendal arteryRat

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Area of Science:

  • Pharmacology
  • Urology
  • Biochemistry

Background:

  • Phosphodiesterase type-5 inhibitors are first-line treatments for erectile dysfunction (ED), but are ineffective for 30-40% of patients.
  • Hydrogen sulfide (H2S) can compensate for nitric oxide (NO) deficiency and improve erectile function.
  • Osthole's potential role in modulating H2S production for smooth muscle relaxation in penile tissues was unexplored.

Purpose of the Study:

  • To investigate the effects of osthole on H2S-producing enzyme expression and H2S production in erectile tissues.
  • To evaluate the contribution of H2S to osthole-induced vascular relaxation in penile arteries and erectile tissues.
  • To assess osthole's efficacy in improving erectile function in a rat model.

Main Methods:

  • Western blot and H2S microsensor experiments were used to measure H2S-producing enzyme expression and H2S production.
  • Myography was performed on mouse corpus cavernosum (MCC) and rat pudendal artery (RPA) to assess vascular relaxation.
  • Intracavernosal pressure measurements in rats were used to evaluate osthole's effect on erectile function.

Main Results:

  • Osthole increased H2S synthesis and H2S-producing enzyme expression in murine erectile tissues, leading to relaxation.
  • Osthole induced relaxation in the rat pudendal artery while simultaneously increasing luminal H2S levels.
  • Osthole enhanced erectile responses in rats, indicating improved erectile function.

Conclusions:

  • Hydrogen sulfide (H2S) plays a significant role in osthole-induced relaxation of penile tissues.
  • Osthole may be a promising therapeutic candidate for treating erectile dysfunction (ED), especially in cases of endothelial dysfunction.
  • Simultaneous measurement of endogenous H2S and vascular contractility provides novel insights into ED mechanisms.